ArticlePlanta2018
Regulation of enzyme activities in carnivorous pitcher plants of the genus Nepenthes.
Article in Planta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- The liquid from carnivorous marsh pitcher plants (Heliamphora sp.) contains endogenous enzymes for prey digestion.Planta · 2026Article
- Transcriptomic prey-capture responses in convergently evolved carnivorous pitcher plants.The New phytologist · 2026Article
- Carnivorous Plants as Model Species for Recent and Further Studies.Results and problems in cell differentiation · 2026Review
- The Localization of Cell Wall Components in the Whole-Mount ImmunolabeledInternational journal of molecular sciences · 2025Article
- Construction costs and tradeoffs in carnivorous pitcher plant leaves: towards a pitcher leaf economics spectrum.Annals of botany · 2025Review
- Recruitment of Sugar Transport and Scent Volatile Genes for Prey Attraction in the Nectar Spoon of Heliamphora tatei.Evolution & development · 2025Article
- The carnivorous rainbow plant Byblis filifolia Planch. secretes digestive enzymes in response to prey capture independently of jasmonates.Plant biology (Stuttgart, Germany) · 2025Article
- Complex transcription regulation of acidic chitinase suggests fine-tuning of digestive processes in Drosera binata.Planta · 2025Article
- Water Cannot Activate Traps of the Carnivorous Sundew PlantPlants (Basel, Switzerland) · 2023Article
- Acid waters in tank bromeliads: Causes and potential consequences.American journal of botany · 2023Article
- The digestive systems of carnivorous plants.Plant physiology · 2022Review
- Alternative oxidase (AOX) in the carnivorous pitcher plants of the genus Nepenthes: what is it good for?Annals of botany · 2022Article
- Bacterial Recruitment to Carnivorous Pitcher Plant Communities: Identifying Sources Influencing Plant Microbiome Composition and Function.Frontiers in microbiology · 2022Article
- Signaling and transport processes related to the carnivorous lifestyle of plants living on nutrient-poor soil.Plant physiology · 2021Review
- Review
- A comparative UHPLC-Q/TOF-MS-based eco-metabolomics approach reveals temperature adaptation of four Nepenthes species.Scientific reports · 2020Article
- Investigation of an Elevational Gradient Reveals Strong Differences Between Bacterial and Eukaryotic Communities Coinhabiting Nepenthes Phytotelmata.Microbial ecology · 2020Article
- Jasmonate-independent regulation of digestive enzyme activity in the carnivorous butterwort Pinguicula × Tina.Journal of experimental botany · 2020Article
- Molecular Properties and New Potentials of Plant Nepenthesins.Plants (Basel, Switzerland) · 2020Review
- Transcriptome-wide shift from photosynthesis and energy metabolism upon endogenous fluid protein depletion in young Nepenthes ampullaria pitchers.Scientific reports · 2020Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
MAIN
conclusionNepenthes regulates enzyme activities by sensing stimuli from the insect prey. Protein is the best inductor mimicking the presence of an insect prey. Carnivorous plants of the genus Nepenthes have evolved passive pitcher traps for prey capture. In this study, we investigated the ability of chemical signals from a prey (chitin, protein, and ammonium) to induce transcription and synthesis of digestive enzymes in Nepenthes × Mixta. We used real-time PCR and specific antibodies generated against the aspartic proteases nepenthesins, and type III and type IV chitinases to investigate the induction of digestive enzyme synthesis in response to different chemical stimuli from the prey. Transcription of nepenthesins was strongly induced by ammonium, protein and live prey; chitin induced transcription only very slightly. This is in accordance with the amount of released enzyme and proteolytic activity in the digestive fluid. Although transcription of type III chitinase was induced by all investigated stimuli, a significant accumulation of the enzyme in the digestive fluid was found mainly after protein and live prey addition. Protein and live prey were also the best inducers for accumulation of type IV chitinase in the digestive fluid. Although ammonium strongly induced transcription of all investigated genes probably through membrane depolarization, strong acidification of the digestive fluid affected stability and abundance of both chitinases in the digestive fluid. The study showed that the proteins are universal inductors of enzyme activities in carnivorous pitcher plants best mimicking the presence of insect prey. This is not surprising, because proteins are a much valuable source of nitrogen, superior to chitin. Extensive vesicular activity was observed in prey-activated glands.
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