ArticleAnimal nutrition (Zhongguo xu mu shou yi xue hui)2015
The profiles of mitochondrial respiration and glycolysis using extracellular flux analysis in porcine enterocyte IPEC-J2.
Article in Animal nutrition (Zhongguo xu mu shou yi xue hui), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Rewriting nuclear epigenetic scripts in mitochondrial diseases as a strategy for heteroplasmy control.EMBO molecular medicine · 2025Article
- Impacts of 360 mg/kg Niacinamide Supplementation in Low-Protein Diets on Energy and Nitrogen Metabolism and Intestinal Microbiota in Growing-Finishing Pigs.Animals : an open access journal from MDPI · 2025Article
- Potential probiotic Lactiplantibacillus plantarum strains alleviate TNF-α by regulating ADAM17 protein and ameliorate gut integrity through tight junction protein expression in in vitro model.Cell communication and signaling : CCS · 2024Article
- The microtubule targeting agent ST-401 triggers cell death in interphase and prevents the formation of polyploid giant cancer cells.Journal of translational medicine · 2024Article
- Nutritional Composition and Pharmacological Activity of Musa balbisiana Colla Seed: An Insight into Phytochemical and Cellular Bioenergetic Profiling.Plant foods for human nutrition (Dordrecht, Netherlands) · 2023Article
- A multi-omics longitudinal aging dataset in primary human fibroblasts with mitochondrial perturbations.Scientific data · 2022Article
- TNFα-Induced Oxidative Stress and Mitochondrial Dysfunction Alter Hypothalamic Neurogenesis and Promote Appetite Versus Satiety Neuropeptide Expression in Mice.Brain sciences · 2022Article
- Impact of experimental colitis on mitochondrial bioenergetics in intestinal epithelial cells.Scientific reports · 2022Article
- AMPK-PINK1/Parkin Mediated Mitophagy Is Necessary for Alleviating Oxidative Stress-Induced Intestinal Epithelial Barrier Damage and Mitochondrial Energy Metabolism Dysfunction in IPEC-J2.Antioxidants (Basel, Switzerland) · 2021Article
- Insights into Cadmium-Induced Carcinogenesis through an In Vitro Study Using C3H10T1/2Cl8 Cells: The Multifaceted Role of Mitochondria.International journal of molecular sciences · 2021Article
- Mitochondrial and glycolytic extracellular flux analysis optimization for isolated pig intestinal epithelial cells.Scientific reports · 2021Article
- PlGF knockdown attenuates hypoxia-induced stimulation of cell proliferation and glycolysis of lung adenocarcinoma through inhibiting Wnt/β-catenin pathway.Cancer cell international · 2021Article
- Vitamin K Vitamers Differently Affect Energy Metabolism in IPEC-J2 Cells.Frontiers in molecular biosciences · 2021Article
- TNFAIP8 drives metabolic reprogramming to promote prostate cancer cell proliferation.The international journal of biochemistry & cell biology · 2021Article
- ATG12 deficiency leads to tumor cell oncosis owing to diminished mitochondrial biogenesis and reduced cellular bioenergetics.Cell death and differentiation · 2020Article
- Romo1 is involved in the immune response of glioblastoma by regulating the function of macrophages.Aging · 2020Article
- Acute psychological stress increases serum circulating cell-free mitochondrial DNA.Psychoneuroendocrinology · 2019Article
- Systems biology identifies preserved integrity but impaired metabolism of mitochondria due to a glycolytic defect in Alzheimer's disease neurons.Aging cell · 2019Article
- Astrocytes in juvenile neuronal ceroid lipofuscinosis (CLN3) display metabolic and calcium signaling abnormalities.Journal of neurochemistry · 2019Article
- Ethyl Pyruvate Modulates Murine Dendritic Cell Activation and Survival Through Their Immunometabolism.Frontiers in immunology · 2019Article
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5 authors.
Funding
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Abstract
The porcine intestinal mucosa require large amounts of energy for nutrient processing and cellular functions and is vulnerable to injury by weaning stress involving bioenergetics failure. The mitochondrial bioenergetic measurement in porcine enterocytes have not been defined. The present study was to establish a method to measure mitochondrial respiratory function and profile mitochondrial function of IPEC-J2 using cell mito stress test and glycolysis stress test assay by XF24 extracellular flux analyzer. The optimal seeding density and concentrations of the injection compounds were determined to be 40,000 cells/well as well as 0.5 µM oligomycin, 1 µM carbonyl cyanide p-trifluoromethoxy-phenylhydrazone (FCCP) and 1 µM rotenone & antimycin A, respectively. The profiles of mitochondrial respiration and glycolysis confirmed that porcine enterocyte preferentially derived much more energy from glutamine than glucose. These results will provide a basis for further study of mitochondrial function and bioenergetics of the porcine small intestine.
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