ReviewAngiogenesis2018
Consensus guidelines for the use and interpretation of angiogenesis assays.
Open access · hybridAbstract readReview
In one paragraph
Review in Angiogenesis, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 373 papers, 1 of them a synthesis that pooled it.
0numbers the graph read from it
0cells of the map it votes in
373citing papers in PubMed, 1 pooled it
25.9field-weighted citation impact, top 1% of its field
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
373 citing papers in PubMed, 1 synthesis or guideline pooled it, 588 citations in OpenAlex.
- Modification of titanium orthopedic implants with bioactive glass: a systematic review ofFrontiers in bioengineering and biotechnology · 2023Pooled it
- Linking preclinical models with functional imaging and artificial intelligence to decode tumor angiogenesis.Pharmacological reports : PR · 2026Review
- Endothelial Cell-Pericyte Crosstalk in the Brain Balances Microvascular Stability and Plasticity.Arteriosclerosis, thrombosis, and vascular biology · 2026Review
- Workflow evaluation of a commercial deep learning-based image analysis tool for the quantification of angiogenesis in vivo.Scientific reports · 2026Article
- Article
- Article
- 3D co-culture model of tumor spheroid and endothelial cells unveils ccRCC aberrant vasculature and distinct sensitivity to targeted treatments.Angiogenesis · 2026Article
- Article
- Deciphering Colorectal Liver Metastasis Perfusion Heterogeneity: Identification of Distinct Vascular Phenotypes through High-Lateral-Resolution Photoacoustic Microscopy.Radiology. Imaging cancer · 2026Article
- Prevascularized grafts with spatially organized MSC spheroids to accelerate therapeutic angiogenesis in ischemic disease.Angiogenesis · 2026Article
- Spatial distribution and density of fibroblasts determine angiogenic response of endothelial cells.Research square · 2026Article
- Article
- CRISPR and compound screens in a novel ex vivo tissue model identify DDR1 and ETA as regulators of cancer cell invasion.Cellular & molecular biology letters · 2026Article
- The HCMV-encoded miR-UL36-3p promotes angiogenesis of endothelial cells by downregulating FOXO3.Animal models and experimental medicine · 2026Article
- Human MAGI1 expression in endothelial cells protects from the development of localized and systemic scleroderma in mice.Arthritis research & therapy · 2026Article
- Fibrin scaffolds for angiogenesis in soft tissue models: a systematic review.Bioactive materials · 2026Review
- Boosting angiogenesis experimentally in ovo by biofunctionalizing collagen membranes with platelet-rich fibrin and hyaluronic acid: implications for regenerative oral surgery?International journal of implant dentistry · 2026Article
- Combined treatment using repurposed synthetic peptide desmopressin and bevacizumab as a potential antiangiogenic strategy in osteosarcoma.Frontiers in medicine · 2026Article
- Metabolic Symbiosis Between Cancer Cells and Endothelial Cells: A Key Driver of Tumor Angiogenesis.Advances in experimental medicine and biology · 2026Review
- Evaluation of Anti-angiogenic Activity of the Acetone Leaf Extract ofActa medica Philippina · 2026Article
313 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
86 authors at 20 institutions in 14 countries.
Patrycja Nowak-SliwinskaMolecular Pharmacology Group, School of Pharmaceutical Sciences, Faculty of Sciences, University of Geneva, University of Lausanne, Rue Michel-Servet 1, CMU, 1211, Geneva 4, Switzerland. Patrycja.Nowak-Sliwinska@unige.ch.
Kari AlitaloWihuri Research Institute and Translational Cancer Biology Program, University of Helsinki, Helsinki, Finland.
Elizabeth AllenLaboratory of Tumor Microenvironment and Therapeutic Resistance, Department of Oncology, VIB-Center for Cancer Biology, KU Leuven, Louvain, Belgium.
Andrey AnisimovWihuri Research Institute and Translational Cancer Biology Program, University of Helsinki, Helsinki, Finland.
Alfred C AplinDepartment of Pathology, University of Washington, Seattle, WA, USA.
Robert AuerbachUniversity of Wisconsin, Madison, WI, USA.
Hellmut G AugustinEuropean Center for Angioscience, Medical Faculty Mannheim, Heidelberg University, Heidelberg, Germany.
David O BatesDivision of Cancer and Stem Cells, School of Medicine, University of Nottingham, Nottingham, UK.
Judy R van BeijnumAngiogenesis Laboratory, Department of Medical Oncology, VU University Medical Center, Cancer Center Amsterdam, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
R Hugh F BenderDepartment of Molecular Biology and Biochemistry, University of California, Irvine, CA, USA.
Gabriele BergersLaboratory of Tumor Microenvironment and Therapeutic Resistance, Department of Oncology, VIB-Center for Cancer Biology, KU Leuven, Louvain, Belgium.
Andreas BikfalviAngiogenesis and Tumor Microenvironment Laboratory (INSERM U1029), University Bordeaux, Pessac, France.
Joyce BischoffVascular Biology Program and Department of Surgery, Harvard Medical School, Boston Children's Hospital, Boston, MA, USA.
Barbara C BöckEuropean Center for Angioscience, Medical Faculty Mannheim, Heidelberg University, Heidelberg, Germany.
Peter C BrooksCenter for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, ME, USA.
Federico BussolinoDepartment of Oncology, University of Torino, Turin, Italy.
Bertan CakirDepartment of Ophthalmology, Harvard Medical School, Boston Children's Hospital, Boston, MA, USA.
Peter CarmelietLaboratory of Angiogenesis and Vascular Metabolism, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
Daniel CastranovaDivision of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
Anca M CimpeanDepartment of Microscopic Morphology/Histology, Angiogenesis Research Center, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania.
Ondine CleaverDepartment of Molecular Biology, Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA.
George CoukosLudwig Institute for Cancer Research, Department of Oncology, University of Lausanne, Lausanne, Switzerland.
George E DavisDepartment of Medical Pharmacology and Physiology, University of Missouri, School of Medicine and Dalton Cardiovascular Center, Columbia, MO, USA.
Michele De PalmaSchool of Life Sciences, Swiss Federal Institute of Technology, Lausanne, Switzerland.
Anna DimbergDepartment of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.
Ruud P M DingsDepartment of Radiation Oncology, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
Valentin DjonovInstitute of Anatomy, University of Bern, Bern, Switzerland.
Andrew C DudleyDepartment of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA.
Neil P DuftonVascular Sciences, Imperial Centre for Translational and Experimental Medicine, National Heart and Lung Institute, Imperial College London, London, UK.
Sarah-Maria FendtLaboratory of Cellular Metabolism and Metabolic Regulation, VIB Center for Cancer Biology, Leuven, Belgium.
Napoleone FerraraUniversity of California, San Diego, La Jolla, CA, USA.
Marcus FruttigerInstitute of Ophthalmology, University College London, London, UK.
Dai FukumuraEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
Bart GhesquièreMetabolomics Expertise Center, VIB Center for Cancer Biology, VIB, Leuven, Belgium.
Yan GongDepartment of Ophthalmology, Harvard Medical School, Boston Children's Hospital, Boston, MA, USA.
Robert J GriffinDepartment of Radiation Oncology, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
Adrian L HarrisMolecular Oncology Laboratories, Oxford University Department of Oncology, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
Christopher C W HughesDepartment of Molecular Biology and Biochemistry, University of California, Irvine, CA, USA.
Nan W HultgrenDepartment of Molecular Biology and Biochemistry, University of California, Irvine, CA, USA.
M Luisa Iruela-ArispeMCDB, University of California, Los Angeles, CA, USA.
Melita IrvingLudwig Institute for Cancer Research, Department of Oncology, University of Lausanne, Lausanne, Switzerland.
Rakesh K JainEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
Raghu KalluriDepartment of Cancer Biology, Metastasis Research Center, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Joanna KaluckaLaboratory of Angiogenesis and Vascular Metabolism, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
Robert S KerbelDepartment of Medical Biophysics, Biological Sciences Platform, Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada.
Jan KitajewskiDepartment of Physiology and Biophysics, University of Illinois, Chicago, IL, USA.
Ingeborg KlaassenOcular Angiogenesis Group, Departments of Ophthalmology and Medical Biology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
Hynda K KleinmannThe George Washington University School of Medicine, Washington, DC, USA.
Pieter KoolwijkDepartment of Ophthalmology, University of Lausanne, Jules-Gonin Eye Hospital, Fondation Asile des Aveugles, Lausanne, Switzerland.
Elisabeth KuczynskiDepartment of Medical Biophysics, Biological Sciences Platform, Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada.
Brenda R KwakDepartment of Pathology and Immunology, University of Geneva, Geneva, Switzerland.
Koen MarienHistoGeneX, Antwerp, Belgium.
Juan M Melero-MartinDepartment of Cardiac Surgery, Harvard Medical School, Boston Children's Hospital, Boston, MA, USA.
Lance L MunnEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
Roberto F NicosiaDepartment of Pathology, University of Washington, Seattle, WA, USA.
Agnes NoelLaboratory of Tumor and Developmental Biology, GIGA-Cancer, University of Liège, Liège, Belgium.
Jussi NurroDepartment of Biotechnology and Molecular Medicine, University of Eastern Finland, Kuopio, Finland.
Anna-Karin OlssonDepartment of Medical Biochemistry and Microbiology, Science for Life Laboratory, Uppsala Biomedical Center, Uppsala University, Uppsala, Sweden.
Tatiana V PetrovaDepartment of oncology UNIL-CHUV, Ludwig Institute for Cancer Research Lausanne, Lausanne, Switzerland.
Kristian PietrasDivision of Translational Cancer Research, Department of Laboratory Medicine, Lund, Sweden.
Roberto PiliGenitourinary Program, Indiana University-Simon Cancer Center, Indianapolis, IN, USA.
Jeffrey W PollardMedical Research Council Centre for Reproductive Health, College of Medicine and Veterinary Medicine, University of Edinburgh, Edinburgh, UK.
Mark J PostDepartment of Physiology, Maastricht University, Maastricht, The Netherlands.
Paul H A QuaxEinthoven Laboratory for Experimental Vascular Medicine, Department Surgery, LUMC, Leiden, The Netherlands.
Gabriel A RabinovichLaboratory of Immunopathology, Institute of Biology and Experimental Medicine, National Council of Scientific and Technical Investigations (CONICET), Buenos Aires, Argentina.
Marius RaicaDepartment of Microscopic Morphology/Histology, Angiogenesis Research Center, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania.
Anna M RandiVascular Sciences, Imperial Centre for Translational and Experimental Medicine, National Heart and Lung Institute, Imperial College London, London, UK.
Domenico RibattiDepartment of Basic Medical Sciences, Neurosciences and Sensory Organs, University of Bari Medical School, Bari, Italy.
Curzio RueggDepartment of Oncology, Microbiology and Immunology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland.
Reinier O SchlingemannOcular Angiogenesis Group, Departments of Ophthalmology and Medical Biology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
Stefan Schulte-MerkerInstitute of Cardiovascular Organogenesis and Regeneration, Faculty of Medicine, WWU, Münster, Germany.
Lois E H SmithDepartment of Ophthalmology, Harvard Medical School, Boston Children's Hospital, Boston, MA, USA.
Jonathan W SongDepartment of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH, USA.
Steven A StackerTumour Angiogenesis and Microenvironment Program, Peter MacCallum Cancer Centre and The Sir Peter MacCallum, Department of Oncology, University of Melbourne, Melbourne, VIC, Australia.
Jimmy StalinInstitute of Cardiovascular Organogenesis and Regeneration, Faculty of Medicine, WWU, Münster, Germany.
Amber N StratmanDivision of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
Maureen Van de VeldeLaboratory of Tumor and Developmental Biology, GIGA-Cancer, University of Liège, Liège, Belgium.
Victor W M van HinsberghDepartment of Ophthalmology, University of Lausanne, Jules-Gonin Eye Hospital, Fondation Asile des Aveugles, Lausanne, Switzerland.
Peter B VermeulenHistoGeneX, Antwerp, Belgium.
Johannes WaltenbergerMedical Faculty, University of Münster, Albert-Schweitzer-Campus 1, Münster, Germany.
Brant M WeinsteinDivision of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
Hong XinUniversity of California, San Diego, La Jolla, CA, USA.
Bahar Yetkin-ArikOcular Angiogenesis Group, Departments of Ophthalmology and Medical Biology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
Seppo Yla-HerttualaDepartment of Biotechnology and Molecular Medicine, University of Eastern Finland, Kuopio, Finland.
Mervin C YoderDepartment of Pediatrics, Indiana University School of Medicine, Indianapolis, IN, USA.
Arjan W GriffioenAngiogenesis Laboratory, Department of Medical Oncology, VU University Medical Center, Cancer Center Amsterdam, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands. aw.griffioen@vumc.nl.
Boston Children's Hospital · USHarvard University · USKU Leuven · BEUniversity of California, Irvine · USUniversity of Lausanne · CHAmsterdam UMC Location University of Amsterdam · NLAmsterdam UMC Location Vrije Universiteit Amsterdam · NLHistoGeneX (Belgium) · BEImperial College London · GBIndiana University – Purdue University Indianapolis · USMassachusetts General Hospital · USNational Institutes of Health · USUniversity of Arkansas for Medical Sciences · USUniversity of California San Diego · USUniversity of Eastern Finland · FIUniversity of Geneva · CHUniversity of Liège · BEUniversity of Toronto · CAUniversity of Washington · USUppsala University · SE
Funding
Women's CancersP30CA016042 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Samuel Wheeler French · 1985 to 2026
$134.5MVascular Normalization: Rolse of Perivascular CellsP01CA080124 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI JAIN, RAKESH K. · 2000 to 2016
$35.0MSpecification And Patterning of Developing Blood VesselsZIAHD001011 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI WEINSTEIN, BRANT · 2009 to 2025
$18.4MDietary control of angiogenesis in retinopathy modelsR01EY017017 · NEI · BOSTON CHILDREN'S HOSPITAL · PI Zhongjie Fu, Lois Smith · 2006 to 2026
$11.8MRegulation of Vascular IntegrityZIAHD008915 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI WEINSTEIN, BRANT · 2011 to 2025
$9.2MNovel therapies to inhibit Diabetic RetinopathyR24EY024864 · NEI · UNIVERSITY OF CALIFORNIA-IRVINE · PI KERN, TIMOTHY S · 2015 to 2019
$8.1MBlood vessel assembly from multipotent hemangioma-derived stem cellsR01HL096384 · NHLBI · BOSTON CHILDREN'S HOSPITAL · PI Joyce E. Bischoff · 2009 to 2026
$7.7MNotch in Angiogenesis and Vascular BiologyR01HL112626 · NHLBI · UNIVERSITY OF ILLINOIS AT CHICAGO · PI Jan K. Kitajewski · 2012 to 2026
$6.3MDissecting Pediatric Brain Tumor Microenvironment to Improve TreatmentR35CA197743 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI JAIN, RAKESH K. · 2015 to 2020
$5.7MCapillary malformation: From somatic GNAQ mutations to disrupted endothelial biologyR01HL127030 · NHLBI · BOSTON CHILDREN'S HOSPITAL · PI BISCHOFF, JOYCE E., GREENE, ARIN K. · 2016 to 2023
$5.2MMouse Neurodevelopmental Behavior CoreU54HD090255 · NICHD · BOSTON CHILDREN'S HOSPITAL · PI CHEN, CHINFEI · 2016 to 2020
$5.2MVascular networks genetically engineered for protein drug deliveryR01HL128452 · NHLBI · BOSTON CHILDREN'S HOSPITAL · PI MELERO-MARTIN, JUAN M, WANG, BO · 2015 to 2024
$4.8MBritish Heart Foundation FS/10/47/28393British Heart Foundation PG/09/096/28114British Heart Foundation PG/10/94/28651British Heart Foundation PG/16/91/32515British Heart Foundation RG/11/17/29256British Heart Foundation RG/17/4/32662Cancer Research UK 18974Medical Research Council MR/K015710/1Medical Research Council MR/N022556/1NCI NIH HHS P01 CA080124NCI NIH HHS P30 CA016042NCI NIH HHS R01 CA188404NCI NIH HHS R01 CA201537NCI NIH HHS R01 CA208205NCI NIH HHS R35 CA197743NEI NIH HHS R01 EY017017NEI NIH HHS R24 EY024864NHLBI NIH HHS R01 HL096384NHLBI NIH HHS R01 HL112626NHLBI NIH HHS R01 HL119043NHLBI NIH HHS R01 HL127030NHLBI NIH HHS R01 HL128168NHLBI NIH HHS R01 HL128452NHLBI NIH HHS R01 HL136139NIAID NIH HHS R21 AI123883NIAMS NIH HHS R01 AR069038NICHD NIH HHS U54 HD090255
Abstract
The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation. As such, this collaborative work is the first edition of consensus guidelines on angiogenesis bioassays to serve for current and future reference.
Indexed as
NeoplasmsNeovascularization, PathologicAnimalsBiological AssayGuidelines as TopicHumansMiceAngiogenesisAortic ringChorioallantoic membrane (CAM)Corneal angiogenesisEndothelial cell migrationHindlimb ischemiaIntussusceptive angiogenesisMicrofluidicMyocardial angiogenesisPlug assayProliferationRecombinant proteinsRetinal vasculatureTip cellsVascular networkVessel co-optionZebrafish
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