ArticleOxidative medicine and cellular longevity2018
Effect of Heat Stress on Sperm DNA: Protamine Assessment in Ram Spermatozoa and Testicle.
Article in Oxidative medicine and cellular longevity, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 61 citations in OpenAlex.
- Heat stress-mediated oxidative damage in male germ cells: potential protective effects of L-citrulline.Frontiers in endocrinology · 2026Review
- Optimizing Cryopreservation: The Effect of MitoTEMPO and Glycerol Ratios on Ram Semen Quality After Freezing and Thawing.Veterinary medicine and science · 2025Article
- Potential therapeutic effects of shrimp protein hydrolysates on NAFLD-induced infertility disorders: Insights into redox balance, heat shock protein expression, and chromatin compaction in male rats.Iranian journal of basic medical sciences · 2025Article
- Immediate and late effects of long-term testicular heat stress on the number of seminiferous tubules and cellular content in Santa Inês rams.Animal reproduction · 2025Article
- Effects of heat stress on reproduction and gene expression in sheep.Animal reproduction · 2025Review
- Application of Flow Cytometry Using Advanced Chromatin Analyses for Assessing Changes in Sperm Structure and DNA Integrity in a Porcine Model.International journal of molecular sciences · 2024Article
- The characterization of CellROX™ probes could be a crucial factor in ram sperm quality assessment.Frontiers in veterinary science · 2024Article
- Sperm Chromatin Status and DNA Fragmentation in Mouse Species with Divergent Mating Systems.International journal of molecular sciences · 2023Article
- RNA sequencing profiles reveals progressively reduced spermatogenesis with progression in adult cryptorchidism.Frontiers in endocrinology · 2023Article
- A Case-Control Study on the Effects of Plasticizers Exposure on Male Fertility.International journal of environmental research and public health · 2022Article
- Chronic scrotal heat stress causes testicular interstitial inflammation and fibrosis: An experimental study in mice.International journal of reproductive biomedicine · 2022Article
- Sperm DNA integrity and male infertility: a narrative review and guide for the reproductive physicians.Translational andrology and urology · 2022Review
- The Mechanism of Heat Stress Resistance During Spermatogenesis in Turpan Black Sheep.Frontiers in veterinary science · 2022Article
- What is known so far about bull sperm protamination: a review.Animal reproduction · 2022Review
- Integrated study of circRNA, lncRNA, miRNA, and mRNA networks in mediating the effects of testicular heat exposure.Cell and tissue research · 2021Article
- Effect of Co-exposure to Heat and Psychological Stressors on Sperm DNA and Semen Parameters.Toxicology reports · 2021Article
- Sperm DNA Fragmentation: A New Guideline for Clinicians.The world journal of men's health · 2020Review
- Effect of transient scrotal hyperthermia on human sperm: an iTRAQ-based proteomic analysis.Reproductive biology and endocrinology : RB&E · 2020Article
- Heat stress effects on bovine sperm cells: a chronological approach to early findings.International journal of biometeorology · 2020Article
- High temperature suppressed SSC self-renewal through S phase cell cycle arrest but not apoptosis.Stem cell research & therapy · 2019Article
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sperm DNA fragmentation is considered one of the main causes of male infertility. The most accepted causes of sperm DNA damage are deleterious actions of reactive oxygen species (ROS), defects in protamination, and apoptosis. Ram sperm are highly prone to those damages due to the high susceptibility to ROS and to oxidative stress caused by heat stress. We aimed to evaluate the effects of heat stress on the chromatin of ejaculated and epididymal sperm and the activation of apoptotic pathways in different cell types in ram testis. We observed higher percentages of ejaculated sperm with increased chromatin fragmentation in the heat stress group; a fact that was unexpectedly not observed in epididymal sperm. Heat stress group presented a higher percentage of spermatozoa with DNA fragmentation and increased number of mRNA copies of transitional protein 1. Epididymal sperm presented greater gene expression of protamine 1 on the 30th day of the spermatic cycle; however, no differences in protamine protein levels were observed in ejaculated sperm and testis. Localization of proapoptotic protein BAX or BCL2 in testis was not different. In conclusion, testicular heat stress increases ram sperm DNA fragmentation without changes in protamination and apoptotic patterns.
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