ArticleScientific reports2018
Detection of malaria sporozoites expelled during mosquito sugar feeding.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Effects of Plasmodium berghei infection on the expression of salivary gland immune-related genes in the Anopheles stephensi mosquito.Malaria journal · 2025Article
- Characterization of bacteria expectorated during forced salivation of the Phlebotomus papatasi: A neglected component of sand fly infectious inoculums.PLoS neglected tropical diseases · 2024Article
- Quantification of sporozoite expelling byeLife · 2024Article
- Testing a non-destructive assay to track Plasmodium sporozoites in mosquitoes over time.Parasites & vectors · 2023Article
- Nondestructive Methods of Pathogen Detection: Importance of Mosquito Integrity in Studies of Disease Transmission and Control.Pathogens (Basel, Switzerland) · 2023Review
- Updating the Data on Malaria Vectors in Malaysia: Protocol for a Scoping Review.JMIR research protocols · 2023Article
- Towards environmental detection of Chagas disease vectors and pathogen.Scientific reports · 2022Article
- Membrane Technology for Rapid Point-of-Care Diagnostics for Parasitic Neglected Tropical Diseases.Clinical microbiology reviews · 2021Article
- A non-destructive sugar-feeding assay for parasite detection and estimating the extrinsic incubation period of Plasmodium falciparum in individual mosquito vectors.Scientific reports · 2021Article
- The use of molecular xenomonitoring for surveillance of mosquito-borne diseases.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- Estimating the extrinsic incubation period of malaria using a mechanistic model of sporogony.PLoS computational biology · 2021Article
- Article
- Malaria surveillance from both ends: concurrent detection of Plasmodium falciparum in saliva and excreta harvested from Anopheles mosquitoes.Parasites & vectors · 2019Article
- Experimental reduction of host Plasmodium infection load affects mosquito survival.Scientific reports · 2019Article
- Effect of tris(2-carboxyethyl)phosphine and tertiary butyl alcohol on the performance of convection polymerase chain reaction.Molecular biology reports · 2019Article
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Authors and funding
8 authors.
Funding
Abstract
Malaria is a severe disease of global importance transmitted by mosquitoes of the genus Anopheles. The ability to rapidly detect the presence of infectious mosquitoes able to transmit malaria is of vital importance for surveillance, control and elimination efforts. Current methods principally rely on large-scale mosquito collections followed by labour-intensive salivary gland dissections or enzyme-linked immunosorbent (ELISA) methods to detect sporozoites. Using forced salivation, we demonstrate here that Anopheles mosquitoes infected with Plasmodium expel sporozoites during sugar feeding. Expelled sporozoites can be detected on two sugar-soaked substrates, cotton wool and Whatman FTA cards, and sporozoite DNA is detectable using real-time PCR. These results demonstrate a simple and rapid methodology for detecting the presence of infectious mosquitoes with sporozoites and highlight potential laboratory applications for investigating mosquito-malaria interactions. Our results indicate that FTA cards could be used as a simple, effective and economical tool in enhancing field surveillance activities for malaria.
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