ArticleBMC pharmacology & toxicology2018
Mineral trioxide aggregate affects cell viability and induces apoptosis of stem cells from human exfoliated deciduous teeth.
Article in BMC pharmacology & toxicology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- MTT-Based Cytotoxicity Assessment of Chitosan- and OcteniseptBiomimetics (Basel, Switzerland) · 2026Article
- MTA does not induce oxidative stress and apoptotic response, or calcium-related cellular stress and preserves extracellular matrix homeostasis.Molecular biology reports · 2026Article
- Effects of Photobiomodulation and MTA on SHED cell viability and differentiation: an in vitro comparative study.Odontology · 2026Article
- Calcium silicate cement with phosphate-based glass for enhanced osteogenic activity and mechanical strength.Clinical oral investigations · 2026Article
- An In Vitro Tube Model for Cell Biocompatibility Study of Capping Materials for Regenerative Endodontics.Journal of endodontics · 2025Article
- Review
- Bioceramics in Endodontics: Limitations and Future Innovations-A Review.Dentistry journal · 2025Review
- Biocompatibility and antimicrobial effect of demineralised dentin matrix hydrogel for dental pulp preservation.Odontology · 2025Article
- Impact of different capping materials extracts on proliferation and osteogenic differentiation of cultured human dental pulp stem cells.Scientific reports · 2025Article
- Histological evaluation of the regenerative potential of a novel photocrosslinkable gelatin-treated dentin matrix hydrogel in direct pulp capping: an animal study.BMC oral health · 2024Article
- Comparative Biocompatibility and Odonto-/Osteogenesis Effects of Hydraulic Calcium Silicate-Based Cements in Simulated Direct and Indirect Approaches for Regenerative Endodontic Treatments: A Systematic Review.Journal of functional biomaterials · 2023Review
- Analysis of Pulp Tissue Viability and Cytotoxicity of Pulp Capping Agents.Journal of clinical medicine · 2023Article
- Article
- MTA-Based Cements: Biocompatibility and Effects on the Gene Expression of Collagen Type 1 and TGF-BioMed research international · 2022Article
- Combined effects of bone morphogenetic protein-7 and mineral trioxide aggregate on the proliferation, migration, and differentiation of human dental pulp stem cells.Journal of applied oral science : revista FOB · 2022Article
- Combination of Dental Capping Agents With LowLevel Laser Therapy Increases the Cell Viability Percent of Stem Cells From Apical Papilla (SCAPs).Journal of lasers in medical sciences · 2022Article
- Calcium silicate-based cements cause environmental stiffness and show diverse potential to induce osteogenesis in human osteoblastic cells.Scientific reports · 2021Article
- Review
- Cytocompatibility and Bioactive Properties of Hydraulic Calcium Silicate-Based Cements (HCSCs) on Stem Cells from Human Exfoliated Deciduous Teeth (SHEDs): A Systematic Review of In Vitro Studies.Journal of clinical medicine · 2020Review
- Differential Gene Expression Changes in Human Primary Dental Pulp Cells Treated with Biodentine and TheraCal LC Compared to MTA.Biomedicines · 2020Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMineral trioxide aggregate (MTA) is widely used for pulp-capping procedures in permanent teeth and as a gold standard material in endodontics. The aim of the study was to investigate the effect of MTA on cell viability and apoptosis when MTA is directly in contact with Stem Cells from Human Exfoliated Deciduous Teeth (SHEDs).
methodsMTA was mixed and coated in the bottom of a 24-well plate. SHEDs collected and cultured from normal exfoliated human deciduous teeth (passages 3-4) were seeded on square cover glasses. The glasses with seeded SHEDs were incubated in the plates with or without MTA coating. They were divided into four groups: MTA direct contact, direct control, MTA indirect contact, and indirect control. After 1, 2 and 3 days of culturing, cell morphology was observed and cell viability was assessed by the WST-1 cell cytotoxicity assay. TUNEL assay, immunofluorescent labeling and western blot analysis were used to study the effects of MTA on SHEDs apoptosis.
resultsMTA impaired cell viability of SHEDs in 1, 2 and 3 days, and the effect of direct contact was more severe. Cell apoptosis with positive Annexin V and TUNEL staining was noted when there was direct contact with MTA. Western blot analysis revealed that Bcl-2 and Bcl-xL decreased after SHEDs were in contact with MTA.
conclusionsThis study shows that direct contact with 1 week post-set MTA significantly decreases the viability of SHEDs and induced cell apoptosis. The results suggest that there is a possible cytotoxic effect of pulp tissue when there is direct contact with MTA. Different responses would be expected due to the strong alkaline characteristics of fresh mixed MTA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.