ArticleVirology journal2018
Characterization of a new apple luteovirus identified by high-throughput sequencing.
Article in Virology journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Article
- Haplotype-phased 'Ottawa 3' genome unravels differential reaction of apple rootstock roots to mixed viral infection.The Plant journal : for cell and molecular biology · 2026Article
- Exotic ambrosia beetles and phytopathogenic fungi associated with rapid apple decline in North Carolina.Journal of economic entomology · 2025Article
- Article
- Managing Plant Viruses in Tissue-Cultured Apple and Grapevine: Strategies for Detection and Eradication.The plant pathology journal · 2025Review
- Unveiling viral threats to temperate pome fruits: characterization, transmission, and sustainable management strategies.Frontiers in microbiology · 2025Review
- Investigating the Role of Viruses in the Rapid Decline of Young Apple Trees in High-Density Orchards in New York.Plants (Basel, Switzerland) · 2024Article
- Article
- Plant virus diversity in bee and pollen samples from apple (Frontiers in plant science · 2024Article
- The Rapid Apple Decline Phenomenon: Current Status and Expected Associated Factors in Korea.The plant pathology journal · 2023Review
- Genomic analyses of a widespread blueberry virus in the United States.Virus research · 2023Article
- The Comparative Root System Architecture of Declining and Non-Declining Trees in Two Apple Orchards in New York.Plants (Basel, Switzerland) · 2023Article
- Article
- Article
- Characterizing the Virome of Apple Orchards Affected by Rapid Decline in the Okanagan and Similkameen Valleys of British Columbia (Canada).Pathogens (Basel, Switzerland) · 2022Article
- High-throughput detection of a large set of viruses and viroids of pome and stone fruit trees by multiplex PCR-based amplicon sequencing.Frontiers in plant science · 2022Article
- A bushel of viruses: Identification of seventeen novel putative viruses by RNA-seq in six apple trees.PloS one · 2020Article
- Independent modulation of individual genomic component transcription and a cis-acting element related to high transcriptional activity in a multipartite DNA virus.BMC genomics · 2019Article
- Potential role of weather, soil and plant microbial communities in rapid decline of apple trees.PloS one · 2019Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
Abstract
background'Rapid Apple Decline' (RAD) is a newly emerging problem of young, dwarf apple trees in the Northeastern USA. The affected trees show trunk necrosis, cracking and canker before collapse in summer. In this study, we discovered and characterized a new luteovirus from apple trees in RAD-affected orchards using high-throughput sequencing (HTS) technology and subsequent Sanger sequencing.
methodsIllumina NextSeq sequencing was applied to total RNAs prepared from three diseased apple trees. Sequence reads were de novo assembled, and contigs were annotated by BLASTx. RT-PCR and 5'/3' RACE sequencing were used to obtain the complete genome of a new virus. RT-PCR was used to detect the virus.
resultsThree common apple viruses and a new luteovirus were identified from the diseased trees by HTS and RT-PCR. Sequence analyses of the complete genome of the new virus show that it is a new species of the genus Luteovirus in the family Luteoviridae. The virus is graft transmissible and detected by RT-PCR in apple trees in a couple of orchards.
conclusionsA new luteovirus and/or three known viruses were found to be associated with RAD. Molecular characterization of the new luteovirus provides important information for further investigation of its distribution and etiological role.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.