ReviewAcademic pathology
A Next-Generation Sequencing Primer-How Does It Work and What Can It Do?
Review in Academic pathology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 95 citations in OpenAlex.
- Leveraging innovative diagnostics as a tool to contain superbugs.Antonie van Leeuwenhoek · 2025Review
- Next-generation sequencing: A powerful multi-purpose tool in cell line development for biologics production.Computational and structural biotechnology journal · 2025Review
- An evaluation of nucleic acid-based molecular methods for the detection of plant viruses: a systematic review.Virusdisease · 2024Review
- Mutation profile in liquid biopsy tested by next generation sequencing in Mexican patients with non-small cell lung carcinoma and its impact on survival.Journal of thoracic disease · 2024Article
- Depth of Sequencing Plays a Determining Role in the Characterization of Phage Display Peptide Libraries by NGS.International journal of molecular sciences · 2023Article
- What Whole Genome Sequencing Has Told Us About Pathogenic Vibrios.Advances in experimental medicine and biology · 2023Review
- Case report: A toxoplasmic encephalitis in an immunocompromised child detected through metagenomic next-generation sequencing.Frontiers in public health · 2023Article
- Toward a More Just System of Care in Molecular Pathology.The Milbank quarterly · 2022Article
- Article
- MRD in ALL: Optimization and Innovations.Current hematologic malignancy reports · 2022Review
- Comparative genomic analysis of esophageal squamous cell carcinoma and adenocarcinoma: New opportunities towards molecularly targeted therapy.Acta pharmaceutica Sinica. B · 2022Review
- DisseminatedAnnals of translational medicine · 2022Article
- A Comprehensive Review of Performance of Next-Generation Sequencing Platforms.BioMed research international · 2022Review
- The structural shift and collaboration capacity in GenBank Networks: A longitudinal study.Quantitative science studies · 2022Article
- PRINCESS: comprehensive detection of haplotype resolved SNVs, SVs, and methylation.Genome biology · 2021Article
- Teaching next-generation sequencing to medical students with a portable sequencing device.Perspectives on medical education · 2021Article
- Diagnostic Process Using Endoscopy for Biliary Strictures: A Narrative Review.Journal of clinical medicine · 2021Review
- Pediatric pharmacogenomics: challenges and opportunities: on behalf of the Sanford Children's Genomic Medicine Consortium.The pharmacogenomics journal · 2021Review
- Case Report: AFrontiers in medicine · 2021Article
- Case Report:Frontiers in cardiovascular medicine · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Next-generation sequencing refers to a high-throughput technology that determines the nucleic acid sequences and identifies variants in a sample. The technology has been introduced into clinical laboratory testing and produces test results for precision medicine. Since next-generation sequencing is relatively new, graduate students, medical students, pathology residents, and other physicians may benefit from a primer to provide a foundation about basic next-generation sequencing methods and applications, as well as specific examples where it has had diagnostic and prognostic utility. Next-generation sequencing technology grew out of advances in multiple fields to produce a sophisticated laboratory test with tremendous potential. Next-generation sequencing may be used in the clinical setting to look for specific genetic alterations in patients with cancer, diagnose inherited conditions such as cystic fibrosis, and detect and profile microbial organisms. This primer will review DNA sequencing technology, the commercialization of next-generation sequencing, and clinical uses of next-generation sequencing. Specific applications where next-generation sequencing has demonstrated utility in oncology are provided.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.