ArticleBiomedical optics express2018
Multiscale nonlinear microscopy and widefield white light imaging enables rapid histological imaging of surgical specimen margins.
Article in Biomedical optics express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 36 citations in OpenAlex.
- Diagnostic accuracy of two-photon fluorescence microscopy in Mohs surgery of basal cell carcinomas.Journal of the American Academy of Dermatology · 2026Article
- Rapid evaluation of muscularis propria in transurethral resection of bladder tumour specimens using non-linear microscopy (NLM).Journal of clinical pathology · 2026Article
- Deep UV laser scanning microscopy with integrated 1050 nm spectral-domain optical coherence tomography for multi-contrast tissue imaging.Biomedical optics express · 2025Article
- High area and volumetric throughput parallel two-photon fluorescence microscopy using silicon photomultiplier arrays.Photonics research · 2025Article
- Vacuum-based specimen holder for rapid surgical specimen imaging.Biomedical optics express · 2025Article
- Real-time histological evaluation of gastrointestinal tissue using non-linear microscopy.Journal of clinical pathology · 2025Article
- Rapid Examination of Nonprocessed Renal Cell Carcinoma Using Nonlinear Microscopy.Archives of pathology & laboratory medicine · 2024Article
- Rapid examination of lung tissues by nonlinear microscopy.American journal of clinical pathology · 2024Article
- Quantitative and comparative assessment of dyes and protocols for rapid ex vivo microscopy of fresh tissues.Scientific reports · 2024Article
- Piston-based specimen holder for rapid surgical and biopsy specimen imaging.Biomedical optics express · 2024Article
- Rapid clearing and imaging of Mohs and melanoma surgical margins using a low-cost tissue processor.Biomedical optics express · 2024Article
- Detection of breast cancer cells by a near-infrared fluorescent probe targeting mitochondrial viscosity.Heliyon · 2023Article
- Toward next-generation endoscopes integrating biomimetic video systems, nonlinear optical microscopy, and deep learning.Biophysics reviews · 2023Review
- Rapid histological imaging of bone without microtome sectioning using nonlinear microscopy.Bone · 2022Article
- High-speed mosaic imaging using scanner-synchronized stage position sampling.Journal of biomedical optics · 2022Article
- Improved microscopy with ultraviolet surface excitation (MUSE) using high-index immersion illumination.Biomedical optics express · 2021Article
- Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers.Scientific reports · 2021Article
- Harnessing non-destructive 3D pathology.Nature biomedical engineering · 2021Review
- Evaluation of silicon photomultipliers for multiphoton and laser scanning microscopy.Journal of biomedical optics · 2019Article
- Ex Vivo Microscopy: A Promising Next-Generation Digital Microscopy Tool for Surgical Pathology Practice.Archives of pathology & laboratory medicine · 2019Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
The ability to histologically assess surgical specimens in real-time is a long-standing challenge in cancer surgery, including applications such as breast conserving therapy (BCT). Up to 40% of women treated with BCT for breast cancer require a repeat surgery due to postoperative histological findings of close or positive surgical margins using conventional formalin fixed paraffin embedded histology. Imaging technologies such as nonlinear microscopy (NLM), combined with exogenous fluorophores can rapidly provide virtual H&E imaging of surgical specimens without requiring microtome sectioning, facilitating intraoperative assessment of margin status. However, the large volume of typical surgical excisions combined with the need for rapid assessment, make comprehensive cellular resolution margin assessment during surgery challenging. To address this limitation, we developed a multiscale, real-time microscope with variable magnification NLM and real-time, co-registered position display using a widefield white light imaging system. Margin assessment can be performed rapidly under operator guidance to image specific regions of interest located using widefield imaging. Using simulated surgical margins dissected from human breast excisions, we demonstrate that multi-centimeter margins can be comprehensively imaged at cellular resolution, enabling intraoperative margin assessment. These methods are consistent with pathology assessment performed using frozen section analysis (FSA), however NLM enables faster and more comprehensive assessment of surgical specimens because imaging can be performed without freezing and cryo-sectioning. Therefore, NLM methods have the potential to be applied to a wide range of intra-operative applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.