ArticleJournal of biological engineering2018
Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology.
Article in Journal of biological engineering, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 93 citations in OpenAlex.
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- Chromoprotein-modified plant pathogenic bacteria: tools for experimental tracking and visualization.Microbiology spectrum · 2026Article
- Genetic Engineering of Lysogenic-Lytic Switch Genes ImprovesInternational journal of molecular sciences · 2026Article
- Deep-mutational scanning libraries using Tiled-Region Exchange mutagenesis.G3 (Bethesda, Md.) · 2026Article
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- UsingACS synthetic biology · 2025Article
- Mobilizable shuttle vectors with fluorescent markers functional across different species of bacteria.Applied and environmental microbiology · 2025Article
- Single component CRISPR-mediated base-editors forBiodesign research · 2025Article
- Colored Proteins Act as Biocolorants inMolecules (Basel, Switzerland) · 2025Article
- Modular DNA Construct Design for High-Throughput Golden Gate Assembly.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A frugal CRISPR kit for equitable and accessible education in gene editing and synthetic biology.Nature communications · 2024Article
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- Tailored UPRE2 variants for dynamic gene regulation in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- From Natural Microbe Screening to Sustained Chitinase Activity in Exogenous Hosts.ACS synthetic biology · 2024Article
- Bacterial defense systems exhibit synergistic anti-phage activity.Cell host & microbe · 2024Article
- Method for plasmid-based antibiotic-free fermentation.Microbial cell factories · 2024Article
- Coexistence of nonfluorescent chromoproteins and fluorescent proteins in massiveFrontiers in physiology · 2024Article
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Corrections and comments
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Authors and funding
15 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCoral reefs are colored by eukaryotic chromoproteins (CPs) that are homologous to green fluorescent protein. CPs differ from fluorescent proteins (FPs) by intensely absorbing visible light to give strong colors in ambient light. This endows CPs with certain advantages over FPs, such as instrument-free detection uncomplicated by ultra-violet light damage or background fluorescence, efficient Förster resonance energy transfer (FRET) quenching, and photoacoustic imaging. Thus, CPs have found utility as genetic markers and in teaching, and are attractive for potential cell biosensor applications in the field. Most near-term applications of CPs require expression in a different domain of life: bacteria. However, it is unclear which of the eukaryotic CP genes might be suitable and how best to assay them.
resultsHere, taking advantage of codon optimization programs in 12 cases, we engineered 14 CP sequences (meffRed, eforRed, asPink, spisPink, scOrange, fwYellow, amilGFP, amajLime, cjBlue, meffBlue, aeBlue, amilCP, tsPurple and gfasPurple) into a palette of
conclusionAvailability of 14 engineered CP genes compared in
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.