ArticleNature communications2018
Hierarchical mechanism of amino acid sensing by the T-box riboswitch.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Functional characterization of dynamic nascent RNA folding ensembles in real time.Science advances · 2026Article
- Directional co-transcriptional folding and pausing create kinetic checkpoints for riboswitch-controlled gene expression.bioRxiv : the preprint server for biology · 2026Article
- tRNA-dependent conformational dynamics and folding coordinate translational regulation by a full-length T-box riboswitch.Nature communications · 2025Article
- Translational T-box riboswitches bind tRNA by modulating conformational flexibility.Nature communications · 2024Article
- A nascent riboswitch helix orchestrates robust transcriptional regulation through signal integration.Nature communications · 2024Article
- Engineering a Lactobacillus Lysine Riboswitch to Dynamically Control Metabolic Pathways for Lysine Production inMicroorganisms · 2024Article
- Structural and dynamic mechanisms for coupled folding and tRNA recognition of a translational T-box riboswitch.Nature communications · 2023Article
- Direct observation of tRNA-chaperoned folding of a dynamic mRNA ensemble.Nature communications · 2023Article
- Review
- Cooperativity and Interdependency between RNA Structure and RNA-RNA Interactions.Non-coding RNA · 2021Review
- Strategies for Covalent Labeling of Long RNAs.Chembiochem : a European journal of chemical biology · 2021Review
- Unboxing the T-box riboswitches-A glimpse into multivalent and multimodal RNA-RNA interactions.Wiley interdisciplinary reviews. RNA · 2020Review
- RNA drug discovery: Conformational restriction enhances specific modulation of the T-box riboswitch function.Bioorganic & medicinal chemistry · 2020Article
- A riboswitch gives rise to multi-generational phenotypic heterogeneity in an auxotrophic bacterium.Nature communications · 2020Article
- Structural basis of amino acid surveillance by higher-order tRNA-mRNA interactions.Nature structural & molecular biology · 2019Article
- Article
- Single-molecule analysis of i-motif within self-assembled DNA duplexes and nanocircles.Nucleic acids research · 2019Article
- An evolving tale of two interacting RNAs-themes and variations of the T-box riboswitch mechanism.IUBMB life · 2019Review
- Probing RNA structure and interaction dynamics at the single molecule level.Methods (San Diego, Calif.) · 2019Review
- Build Your Own Microscope: Step-By-Step Guide for Building a Prism-Based TIRF Microscope.Methods and protocols · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
In Gram-positive bacteria, T-box riboswitches control gene expression to maintain the cellular pools of aminoacylated tRNAs essential for protein biosynthesis. Co-transcriptional binding of an uncharged tRNA to the riboswitch stabilizes an antiterminator, allowing transcription read-through, whereas an aminoacylated tRNA does not. Recent structural studies have resolved two contact points between tRNA and Stem-I in the 5' half of the T-box riboswitch, but little is known about the mechanism empowering transcriptional control by a small, distal aminoacyl modification. Using single-molecule fluorescence microscopy, we have probed the kinetic and structural underpinnings of tRNA binding to a glycyl T-box riboswitch. We observe a two-step mechanism where fast, dynamic recruitment of tRNA by Stem-I is followed by ultra-stable anchoring by the downstream antiterminator, but only without aminoacylation. Our results support a hierarchical sensing mechanism wherein dynamic global binding of the tRNA body is followed by localized readout of its aminoacylation status by snap-lock-based trapping.
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Registered trials
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