Evidence map›Paper›PMID 29760143›Full record

ArticleAntimicrobial agents and chemotherapy2018

Developmental Sensitivity in Schistosoma mansoni to Puromycin To Establish Drug Selection of Transgenic Schistosomes.

Hong-Bin Yan, Michael J Smout, Chuan Ju, Anne E Folley, Danielle E Skinner, Victoria H Mann, Alex Loukas, Wei Hu, Paul J Brindley, Gabriel Rinaldi

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Antimicrobial agents and chemotherapy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.6field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

  1. The Transcriptome ofFrontiers in tropical diseases · 2021
    Article
  2. Review
  3. Biomphalaria glabrata immunity: Post-genome advances.Developmental and comparative immunology · 2020
    Review
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 4 countries.

Hong-Bin YanDepartment of Microbiology, Immunology & Tropical Medicine, and Research Center for the Neglected Diseases of Poverty, School of Medicine and Health Sciences, George Washington University, Washington, DC, USA.
Michael J SmoutCentre for Biodiscovery and Molecular Development of Therapeutics, Australian Institute of Tropical Health and Medicine, James Cook University, Smithfield, Cairns, Queensland, Australia.
Chuan JuDepartment of Microbiology, Immunology & Tropical Medicine, and Research Center for the Neglected Diseases of Poverty, School of Medicine and Health Sciences, George Washington University, Washington, DC, USA.
Anne E FolleyDepartment of Microbiology, Immunology & Tropical Medicine, and Research Center for the Neglected Diseases of Poverty, School of Medicine and Health Sciences, George Washington University, Washington, DC, USA.
Danielle E SkinnerDepartment of Microbiology, Immunology & Tropical Medicine, and Research Center for the Neglected Diseases of Poverty, School of Medicine and Health Sciences, George Washington University, Washington, DC, USA.
Victoria H MannDepartment of Microbiology, Immunology & Tropical Medicine, and Research Center for the Neglected Diseases of Poverty, School of Medicine and Health Sciences, George Washington University, Washington, DC, USA.
Alex LoukasCentre for Biodiscovery and Molecular Development of Therapeutics, Australian Institute of Tropical Health and Medicine, James Cook University, Smithfield, Cairns, Queensland, Australia.
Wei HuKey laboratory of Parasitology and Vector biology, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Shanghai, China.
Paul J BrindleyDepartment of Microbiology, Immunology & Tropical Medicine, and Research Center for the Neglected Diseases of Poverty, School of Medicine and Health Sciences, George Washington University, Washington, DC, USA.
Gabriel RinaldiDepartment of Microbiology, Immunology & Tropical Medicine, and Research Center for the Neglected Diseases of Poverty, School of Medicine and Health Sciences, George Washington University, Washington, DC, USA gr10@sanger.ac.uk.
George Washington University · USAustralian Institute of Tropical Health and Medicine · AUChinese Academy of Agricultural Sciences · CNFudan University · CNWellcome Sanger Institute · GB

Funding

Transduction of Schistosoma mansoni by pseudotyped retrovirusR01AI072773 · NIAID · TULANE UNIVERSITY OF LOUISIANA · PI BRINDLEY, PAUL J · 2007 to 2011
$2.5M
Antibiotic selection for schistosome transgenesisR21AI109532 · NIAID · GEORGE WASHINGTON UNIVERSITY · PI BRINDLEY, PAUL J, RINALDI, JOSE GABRIEL · 2014 to 2015
$436k
NIAID NIH HHS HHSN272201000005CNIAID NIH HHS HHSN272201000005INIAID NIH HHS R01 AI072773NIAID NIH HHS R21 AI109532Wellcome TrustWellcome Trust 107475/Z/15/ZWellcome Trust 206194
6 · The paper itself

Abstract

Schistosomiasis is considered the most important disease caused by helminth parasites, in terms of morbidity and mortality. Tools to facilitate gain- and loss-of-function approaches can be expected to precipitate the discovery of novel interventions, and drug selection of transgenic schistosomes would facilitate the establishment of stable lines of engineered parasites. Sensitivity of developmental stages of schistosomes to the aminonucleoside antibiotic puromycin was investigated. For the schistosomulum and sporocyst stages, viability was quantified by fluorescence microscopy following dual staining with fluorescein diacetate and propidium iodine. By 6 days in culture, the 50% lethal concentration (LC

Indexed as

AnimalsAnimals, Genetically ModifiedFemaleFluoresceinsGenomicsMalePuromycinSchistosoma mansoniSchistosomiasisdiacetylfluoresceinFluoresceinsPuromycinantibiotic susceptibilitydrug selectionfunctional genomicspuromycinschistosomiasis

Identifiers

PMID29760143
PMCPMC6105839
OpenAlexW2803797976

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.