Evidence map›Paper›PMID 29759064›Full record

ArticleNutrition journal2018

Propolis ethanolic extracts reduce adenosine diphosphate induced platelet aggregation determined on whole blood.

Mirza Bojić, Andrea Antolić, Maja Tomičić, Željko Debeljak, Željan Maleš

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nutrition journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
3.6field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 25 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Mirza BojićFaculty of Pharmacy and Biochemistry, Department of Medicinal Chemistry, University of Zagreb, A. Kovačića 1, 10000, Zagreb, Croatia. mbojic@pharma.hr.ORCID 0000-0002-8381-9577
Andrea AntolićFaculty of Science, PDS Biology, University of Zagreb, Rooseveltov trg 6, 10000, Zagreb, Croatia.
Maja TomičićDepartment of Platelet and Leukocyte Immunology, Croatian Institute of Transfusion Medicine, Petrova 6, 10000, Zagreb, Croatia.
Željko DebeljakInstitute of Clinical Laboratory Diagnostics, Osijek University Hospital Center, Josipa Huttlera 4, 31000, Osijek, Croatia.
Željan MalešFaculty of Pharmacy and Biochemistry, Department of Pharmaceutical Botany, University of Zagreb, Schrottova 39, 10000, Zagreb, Croatia.
University of Zagreb · HRCroatian Institute of Transfusion Medicine · HRUniversity of Osijek · HR

Funding

Sveučilište u Zagrebu Z1111
6 · The paper itself

Abstract

backgroundPropolis is a well-known bee product containing more than 2000 identified compounds. It has many beneficial effects on human health that include antibacterial, antiviral, anticancer and hepatoprotective justifying its use as a dietary supplement. Platelet aggregation plays crucial role in thrombus formation that can cause stroke or heart attacks. As cardiovascular diseases, including those caused by thrombus formation, are related to 50% of deaths of Western population, the objective of this study was to determine antiaggregatory activity of propolis on platelet aggregation on the whole blood samples.

methodsTwenty one propolis samples from Southeast Europe were characterized by spectrophotometric methods to determine content of the total flavonoids and phenolic acids. High performance liquid chromatography coupled with diode array detection was used to identify and quantify individual polyphenols. Platelet aggregation was tested by impedance aggregometry on the whole blood samples of ten healthy volunteers.

resultsThe mean content of total polyphenols was 136.14 mg/g and ranged from 59.23 to 277.39 mg/g. Content of total flavonoids ranged between 6.83 and 55.44 mg/g with the mean value of 19.28 mg/g. Percentage of total phenolic acids was in the range 8.79 to 45.67% (mean 26.63%). Minimal antiaggregatory concentration, representing the lowest concentration of propolis extract sample that can cause statistically significant reduction of aggregation, ranged from 5 μM to 10.4 mM. Samples of propolis with lower content of luteolin and higher content of pinocembrin-7-methyleter showed better antiplatelet activity i.e. lower values of minimal antiaggregatory concentration.

conclusionsThis is the first study that shows antiaggregatory potential of propolis ethanolic extracts on the whole blood samples in the low micromolar concentrations suggesting that propolis supplementation may influence platelet aggregation and consequently thrombus formation. Further in vivo studies are needed to confirm the beneficial effects in prevention of cardiovascular diseases.

Indexed as

Adenosine DiphosphateAdultChromatography, High Pressure LiquidEthanolEuropeFemaleFlavonoidsHumansHydroxybenzoatesMaleMiddle AgedPlatelet AggregationPolyphenolsPropolisSpectrophotometryAdenosine DiphosphateEthanolFlavonoidsHydroxybenzoatesphenolic acidPolyphenolsPropolisCardiovascular diseasesFlavonoidsPhenolic acidsPlatelet aggregationPropolisThrombus formation

Identifiers

PMID29759064
PMCPMC5952650
OpenAlexW2805683386

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.