ArticlePLoS neglected tropical diseases2018
Characterization of a Trichinella spiralis putative serine protease. Study of its potential as sero-diagnostic tool.
Article in PLoS neglected tropical diseases, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers, 1 of them a synthesis that pooled it.
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Who cites it
58 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Global prevalence of Trichinella in pigs: A systematic review and meta-analysis.Veterinary medicine and science · 2022Pooled it
- Epidemiology, frost resistance, and emerging species-specific diagnostics for Trichinella nativa: Current evidence and future priorities.Veterinary world · 2026Review
- Insights into the Structural and Proteomic Changes inAnimals : an open access journal from MDPI · 2025Article
- Article
- Comparative immunodiagnostic evaluation of recombinant serine proteases fromVeterinary world · 2025Article
- Assessing diagnostic, vaccine and therapeutic potential of selectedFood and waterborne parasitology · 2025Review
- Characterization of a chitinase from Trichinella spiralis and its immunomodulatory effects on allergic airway inflammation in mice.Parasites & vectors · 2025Article
- Vaccination of mice with Trichinella spiralis C-type lectin elicited the protective immunity and enhanced gut epithelial barrier function.PLoS neglected tropical diseases · 2025Article
- A NovelInternational journal of molecular sciences · 2024Article
- Biological characteristics of a new long-chain fatty acid transport protein 1 from Trichinella spiralis and its participation in lipid metabolism, larval moulting, and development.Veterinary research · 2024Article
- Application of a recombinant novel trypsin from Trichinella spiralis for serodiagnosis of trichinellosis.Parasites & vectors · 2024Article
- Quantitative label-free proteomic analysis of excretory-secretory proteins in different developmental stages of Trichinella spiralis.Veterinary research · 2024Article
- Biological characteristics and functions of a novel glutamate dehydrogenase from Trichinella spiralis.Parasite (Paris, France) · 2024Article
- Galactomannan inhibits Trichinella spiralis invasion of intestinal epithelium cells and enhances antibody-dependent cellular cytotoxicity related killing of larvae by driving macrophage polarization.Parasite (Paris, France) · 2024Article
- A novel trypsin of Trichinella spiralis mediates larval invasion of gut epithelium via binding to PAR2 and activating ERK1/2 pathway.PLoS neglected tropical diseases · 2024Article
- Astacin metalloproteases in human-parasitic nematodes.Advances in parasitology · 2024Review
- Trichinella spiralis galectin binding to toll-like receptor 4 induces intestinal inflammation and mediates larval invasion of gut mucosa.Veterinary research · 2023Article
- Trichinella spiralis cathepsin L induces macrophage M1 polarization via the NF-κB pathway and enhances the ADCC killing of newborn larvae.Parasites & vectors · 2023Article
- Identification of antigens in the Trichinella spiralis extracellular vesicles for serological detection of early stage infection in swine.Parasites & vectors · 2023Article
- Binding of Trichinella spiralis C-type lectin with syndecan-1 on intestinal epithelial cells mediates larval invasion of intestinal epithelium.Veterinary research · 2023Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTrichinellosis is a serious zoonositc parasitosis worldwide. Because its clinical manifestations aren't specific, the diagnosis of trichinellosis is not easy to be made. Trichinella spiralis muscle larva (ML) excretory-secretory (ES) antigens are the most widely applied diagnostic antigens for human trichinellosis, but the major drawback of the ES antigens for assaying anti-Trichinella antibodies is the false negative in the early Trichinella infection period. The aim of this study was to characterize the T. spiralis putative serine protease (TsSP) and to investigate its potential use for diagnosis of trichinellosis. METHODOLOGY/PRINCIPAL
findingsThe full-length TsSP sequence was cloned and expressed, and recombinant TsSP (rTsSP) was purified by Ni-NTA-Sefinose Column. On Western blotting analysis the rTsSP was recognized by T. spiralis-infected mouse serum, and the natural TsSP was identified in T. spiralis ML crude and ES antigens by using anti-rTsSP serum. Expression of TsSP was detected at various T. spiralis developmental stages (newborn larvae, muscle larvae, intestinal infective larvae and adult worms). Immunolocalization identified the TsSP principally in cuticles and stichosomes of the nematode. The sensitivity of rTsSP-ELISA and ES-ELISA was 98.11% (52/53) and 88.68% (47/53) respectively (P > 0.05) when the sera from trichinellosis patients were examined. However, while twenty-one serum samples of trichinellosis patients' sera at 19 days post-infection (dpi) were tested, the sensitivity (95.24%) of rTsSP-ELISA was distinctly higher than 71.43% of ES-ELISA (P < 0.05). The specificity (99.53%) of rTsSP-ELISA was remarkably higher than 91.98% of ES-ELISA (P < 0.01). Only one out of 20 serum samples of cysticercosis patients cross-reacted with the rTsSP. Specific anti-Trichinella IgG in infected mice was first detected by rTsSP-ELISA as soon as 7 dpi and antibody positive rate reached 100% on 10 dpi, whereas the ES-ELISA did not permit detection of 100% of infected mice before 16 dpi.
conclusionsThe rTsSP is a potential early diagnostic antigen for human trichinellosis.
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