Evidence map›Paper›PMID 29755741›Full record

ArticleIUCrJ2018

Sample manipulation and data assembly for robust microcrystal synchrotron crystallography.

Gongrui Guo, Martin R Fuchs, Wuxian Shi, John Skinner, Evanna Berman, Craig M Ogata, Wayne A Hendrickson, Sean McSweeney, Qun Liu

Open access · goldAbstract read
In one paragraph

Article in IUCrJ, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 34 citations in OpenAlex.

  1. Article
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  8. Versatile microporous polymer-based supports for serial macromolecular crystallography.Acta crystallographica. Section D, Structural biology · 2021
    Article
  9. Article
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  11. Article
  12. Low-doseIUCrJ · 2020
    Article
  13. Article
  14. Journal of applied crystallography · 2020
    Article
  15. Article
  16. Article
  17. Article
  18. Making routine native SAD a reality: lessons from beamline X06DA at the Swiss Light Source.Acta crystallographica. Section D, Structural biology · 2019
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Gongrui GuoPhoton Science Directorate, NSLS-II, Brookhaven National Laboratory, Upton, NY 11973, USA.
Martin R FuchsPhoton Science Directorate, NSLS-II, Brookhaven National Laboratory, Upton, NY 11973, USA.ORCID https://orcid.org/0000-0001-9784-0927
Wuxian ShiPhoton Science Directorate, NSLS-II, Brookhaven National Laboratory, Upton, NY 11973, USA.
John SkinnerPhoton Science Directorate, NSLS-II, Brookhaven National Laboratory, Upton, NY 11973, USA.
Evanna BermanBiology Department, Brookhaven National Laboratory, Upton, NY 11973, USA.ORCID https://orcid.org/0000-0001-7351-238X
Craig M OgataGM/CA@APS, X-ray Science Division, Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439, USA.
Wayne A HendricksonDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Sean McSweeneyPhoton Science Directorate, NSLS-II, Brookhaven National Laboratory, Upton, NY 11973, USA.
Qun LiuPhoton Science Directorate, NSLS-II, Brookhaven National Laboratory, Upton, NY 11973, USA.ORCID https://orcid.org/0000-0002-1179-290X
Brookhaven National Laboratory · USArgonne National Laboratory · USColumbia University · US

Funding

Life Science and Biomedical technology Research center at NSLS-II LSBRP41GM111244 · NIGMS · BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB · PI MCSWEENEY, SEAN · 2014 to 2018
$16.4M
Case Center for Synchrotron BiosciencesP30EB009998 · NIBIB · CASE WESTERN RESERVE UNIVERSITY · PI CHANCE, MARK R · 2009 to 2018
$8.4M
Training (303-338)P41GM116799 · NIGMS · NEW YORK STRUCTURAL BIOLOGY CENTER · PI BUCHANAN, SUSAN K. · 2016 to 2020
$7.1M
Proposal for an Administrative Supplement for Equipment Purchases for NIGMS Awardees NOT-GM-18-022R01GM107462 · NIGMS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI HENDRICKSON, WAYNE A. · 2014 to 2021
$3.6M
NIBIB NIH HHS P30 EB009998NIGMS NIH HHS P41 GM111244NIGMS NIH HHS P41 GM116799NIGMS NIH HHS R01 GM107462
6 · The paper itself

Abstract

With the recent developments in microcrystal handling, synchrotron microdiffraction beamline instrumentation and data analysis, microcrystal crystallo-graphy with crystal sizes of less than 10 µm is appealing at synchrotrons. However, challenges remain in sample manipulation and data assembly for robust microcrystal synchrotron crystallography. Here, the development of micro-sized polyimide well-mounts for the manipulation of microcrystals of a few micrometres in size and the implementation of a robust data-analysis method for the assembly of rotational microdiffraction data sets from many microcrystals are described. The method demonstrates that microcrystals may be routinely utilized for the acquisition and assembly of complete data sets from synchrotron microdiffraction beamlines.

Indexed as

data analysismicrocrystalsmicrodiffractionmultiple crystalsradiation damagestructural biologyX-ray crystallography

Identifiers

PMID29755741
PMCPMC5929371
OpenAlexW2799524751

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.