ArticleCell2018
LlamaTags: A Versatile Tool to Image Transcription Factor Dynamics in Live Embryos.
Article in Cell, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
79 citing papers in PubMed.
- Rapid transcriptional response to a dynamic morphogen by time integration.Current biology : CB · 2026Article
- A model for boundary-driven tissue morphogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Coordinated active repression operates via transcription factor cooperativity and multiple inactive promoter states in a developing organism.Nature communications · 2025Article
- Novel Fluorescent and Photoconvertible Fusions Reveal Dorsal Activator Dynamics.bioRxiv : the preprint server for biology · 2025Article
- Article
- Deep learning-based high-resolution time inference for deciphering dynamic gene regulation from fixed embryos.Nature communications · 2025Article
- Cell fate ratios are encoded by transcriptional dynamics in the Drosophila retina.Current biology : CB · 2025Article
- Measuring and manipulating localized translation of erm-1 in the C. elegans embryo.Development (Cambridge, England) · 2025Article
- Recent approaches lead to a deeper understanding of diverse segmentation mechanisms in insects, with a focus on the pair-rule genes.Current opinion in insect science · 2025Review
- Article
- The Mechanics of Building Functional Organs.Cold Spring Harbor perspectives in biology · 2025Review
- DRMY1 promotes robust morphogenesis in Arabidopsis by sustaining the translation of cytokinin-signaling inhibitor proteins.Developmental cell · 2024Article
- Target gene responses differ when transcription factor levels are acutely decreased by nuclear export versus degradation.Development (Cambridge, England) · 2024Article
- Optogenetic dissection of transcriptional repression in a multicellular organism.Nature communications · 2024Article
- Discovery of nanobodies: a comprehensive review of their applications and potential over the past five years.Journal of nanobiotechnology · 2024Review
- Boosting the toolbox for live imaging of translation.RNA (New York, N.Y.) · 2024Article
- Target gene responses differ when transcription factor levels are acutely decreased by nuclear export versus degradation.bioRxiv : the preprint server for biology · 2024Article
- Opticool: Cutting-edge transgenic optical tools.PLoS genetics · 2024Review
- Multifaceted effects on even-skipped transcriptional dynamics upon Krüppel dosage changes.Development (Cambridge, England) · 2024Article
- Introduction of Dicistrovirus IRESs into UAS/SV40-polyA constructs results in premature polyadenylation and strong overexpression of the upstream ORF inbioRxiv : the preprint server for biology · 2023Article
19 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Embryonic cell fates are defined by transcription factors that are rapidly deployed, yet attempts to visualize these factors in vivo often fail because of slow fluorescent protein maturation. Here, we pioneer a protein tag, LlamaTag, which circumvents this maturation limit by binding mature fluorescent proteins, making it possible to visualize transcription factor concentration dynamics in live embryos. Implementing this approach in the fruit fly Drosophila melanogaster, we discovered stochastic bursts in the concentration of transcription factors that are correlated with bursts in transcription. We further used LlamaTags to show that the concentration of protein in a given nucleus heavily depends on transcription of that gene in neighboring nuclei; we speculate that this inter-nuclear signaling is an important mechanism for coordinating gene expression to delineate straight and sharp boundaries of gene expression. Thus, LlamaTags now make it possible to visualize the flow of information along the central dogma in live embryos.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.