ArticlePlant biotechnology journal2019
PbrmiR397a regulates lignification during stone cell development in pear fruit.
Article in Plant biotechnology journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed, 210 citations in OpenAlex.
- The Landscape of Long Non-Coding RNAs Provides Insights Into the Domestication and Improvement of Pear Fruit.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- ARF6 integrates auxin and gibberellin signaling to promote stone cell lignification in pear via the HB49-MYB169 module.The New phytologist · 2026Article
- Cu-miRNA-mediated redox homeostasis explains the differences in cold tolerance between AAA and ABB banana varieties.Journal of advanced research · 2026Article
- Pear scion genotypes shape rhizosphere fungal communities in association with photosynthetic sugar metabolism.BMC plant biology · 2026Article
- The PbrMADS1-PbrMYB169 complex has uniquely emerged to regulate lignification of stone cells in pear.Journal of integrative plant biology · 2026Article
- A Novel ZjbZIP33-ZjPRX1 Module Positively Regulates Lignin Formation in the Jujube Fruit Stone.Plant biotechnology journal · 2025Article
- Haplotype-resolved, gap-free genome assemblies provide insights into the divergence between Asian and European pears.Nature genetics · 2025Article
- XYLEM NAC DOMAIN 1 (EjXND1) relieves cold-induced lignification by negatively regulating the EjHB1-EjPRX12 module in loquat fruit.Journal of advanced research · 2025Article
- CaHorticulture research · 2025Article
- Integrative analyses reveal Bna-miR397a-BnaLAC2 as a potential modulator of low-temperature adaptability in Brassica napus L.Plant biotechnology journal · 2025Article
- What do microRNA concentrations tell us about the mechanical damage and storage period of strawberry fruits?Food chemistry. Molecular sciences · 2025Article
- Nitrate Modulates Fruit Lignification by RegulatingInternational journal of molecular sciences · 2025Article
- Role of non-coding RNAs in quality improvement of horticultural crops: computational tools, databases, and algorithms for identification and analysis.Functional & integrative genomics · 2025Review
- Gap-free genome assemblies of two Pyrus bretschneideri cultivars and GWAS analyses identify a CCCH zinc finger protein as a key regulator of stone cell formation in pear fruit.Plant communications · 2025Article
- Detection of Ethylene Signaling Using Bioinformatics Tools in Plants.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Multi-omics analysis of molecular mechanisms driving the grafting- enhanced resistance of tea plants toFrontiers in plant science · 2025Article
- GhSBI1, a CUP-SHAPED COTYLEDON 2 homologue, modulates branch internode elongation in cotton.Plant biotechnology journal · 2024Article
- TheHorticulture research · 2024Article
- Genome-wide identification of GA2ox genes family and analysis of PbrGA2ox1-mediated enhanced chlorophyll accumulation by promoting chloroplast development in pear.BMC plant biology · 2024Article
- Does Regulated Deficit Irrigation Affect Pear Fruit Texture by Modifying the Stone Cells?Plants (Basel, Switzerland) · 2023Article
11 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Lignified stone cells substantially reduce fruit quality. Therefore, it is desirable to inhibit stone cell development using genetic technologies. However, the molecular mechanisms regulating lignification are poorly understood in fruit stone cells. In this study, we have shown that microRNA (miR) miR397a regulates fruit cell lignification by inhibiting laccase (LAC) genes that encode key lignin biosynthesis enzymes. Transient overexpression of PbrmiR397a, which is the miR397a of Chinese pear (Pyrus bretschneideri), and simultaneous silencing of three LAC genes reduced the lignin content and stone cell number in pear fruit. A single nucleotide polymorphism (SNP) identified in the promoter of the PbrmiR397a gene was found to associate with low levels of fruit lignin, after analysis of the genome sequences of sixty pear varieties. This SNP created a TCA element that responded to salicylic acid to induce gene expression as confirmed using a cell-based assay system. Furthermore, stable overexpression of PbrmiR397a in transgenic tobacco plants reduced the expression of target LAC genes and decreased the content of lignin but did not change the ratio of syringyl- and guaiacyl-lignin monomers. Consistent with reduction in lignin content, the transgenic plants showed fewer numbers of vessel elements and thinner secondary walls in the remaining elements compared to wild-type control plants. This study has advanced our understanding of the regulation of lignin biosynthesis and provided useful molecular genetic information for improving pear fruit quality.
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