ArticleGlycoconjugate journal2018
Cloning, purification and biochemical characterisation of a GH35 beta-1,3/beta-1,6-galactosidase from the mucin-degrading gut bacterium Akkermansia muciniphila.
Article in Glycoconjugate journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Fast structural search for classification of gut bacterial mucin O-glycan degrading enzymes.PLoS computational biology · 2026Article
- Akkermansia muciniphila: promises and pitfallsfor next-generation beneficial microorganisms.Archives of microbiology · 2025Review
- Proteomic study ofFrontiers in microbiology · 2025Article
- Mechanism of 2'-fucosyllactose degradation by human-associatedJournal of bacteriology · 2024Article
- Biochemical Insights into a Novel Family 2 Glycoside Hydrolase with Both β-1,3-Galactosidase and β-1,4-Galactosidase Activity from the Arctic.Marine drugs · 2023Article
- A genetic system for Akkermansia muciniphila reveals a role for mucin foraging in gut colonization and host sterol biosynthesis gene expression.Nature microbiology · 2023Article
- Cloning, Expression, Purification and Characterization of the β-galactosidase PoβGal35A from Penicillium oxalicum.Molecular biotechnology · 2023Article
- Mucin utilization by gut microbiota: recent advances on characterization of key enzymes.Essays in biochemistry · 2023Review
- O-Mucin-degrading carbohydrate-active enzymes and their possible implication in inflammatory bowel diseases.Essays in biochemistry · 2023Review
- Sialidases and fucosidases of Akkermansia muciniphila are crucial for growth on mucin and nutrient sharing with mucus-associated gut bacteria.Nature communications · 2023Article
- Function and Structure ofJournal of applied glycoscience · 2023Article
- How microbial glycosyl hydrolase activity in the gut mucosa initiates microbial cross-feeding.Glycobiology · 2022Review
- A Novel Thermal-Activated β-Galactosidase fromFoods (Basel, Switzerland) · 2022Article
- Microbial Glycoside Hydrolases in the First Year of Life: An Analysis Review on Their Presence and Importance in Infant Gut.Frontiers in microbiology · 2021Review
- Aberration of Serum and Tissue N-Glycans in Mouse β1,4-GalT1 Y286L Mutant Variants.Glycoconjugate journal · 2020Article
- Akkermansia muciniphila uses human milk oligosaccharides to thrive in the early life conditions in vitro.Scientific reports · 2020Article
- Cloning and Characterization of a New β-Galactosidase fromMarine drugs · 2020Article
- SialylatedJournal of applied glycoscience · 2020Article
- Characterization ofInternational journal of molecular sciences · 2019Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
A putative GH35 β-galactosidase gene from the mucin-degrading bacterium Akkermansia muciniphila was successfully cloned and further investigated. The recombinant enzyme with the molecular mass of 74 kDa was purified to homogeneity and biochemically characterised. The optimum temperature of the enzyme was 42 °C, and the optimum pH was determined to be pH 3.5. The addition of sodium dodecyl sulphate (SDS) reduced the enzyme's activity significantly. The addition of Mg
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