Evidence map›Paper›PMID 29754212›Full record

ArticleSupportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer2018

Matrix metalloproteinase expression is altered in the small and large intestine following fractionated radiation in vivo.

Romany L Stansborough, Noor Al-Dasooqi, Emma H Bateman, Joanne M Bowen, Dorothy M K Keefe, Richard M Logan, Ann S J Yeoh, Eric E K Yeoh, Andrea M Stringer, Rachel J Gibson

Abstract read
PubMed Publisher
In one paragraph

Article in Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.1field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Romany L StansboroughAdelaide Medical School, University of Adelaide, North Terrace, Adelaide, 5005, Australia. romany.stansborough@adelaide.edu.au.ORCID http://orcid.org/0000-0001-8302-6449
Noor Al-DasooqiAdelaide Medical School, University of Adelaide, North Terrace, Adelaide, 5005, Australia.
Emma H BatemanAdelaide Medical School, University of Adelaide, North Terrace, Adelaide, 5005, Australia.
Joanne M BowenAdelaide Medical School, University of Adelaide, North Terrace, Adelaide, 5005, Australia.
Dorothy M K KeefeAdelaide Medical School, University of Adelaide, North Terrace, Adelaide, 5005, Australia.
Richard M LoganAdelaide Dental School, University of Adelaide, Adelaide, Australia.
Ann S J YeohAdelaide Medical School, University of Adelaide, North Terrace, Adelaide, 5005, Australia.
Eric E K YeohAdelaide Medical School, University of Adelaide, North Terrace, Adelaide, 5005, Australia.
Andrea M StringerDivision of Health Sciences, University of South Australia, Adelaide, Australia.
Rachel J GibsonAdelaide Medical School, University of Adelaide, North Terrace, Adelaide, 5005, Australia.
The University of Adelaide · AUUniversity of South Australia · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeRadiotherapy-induced gut toxicity (RIGT) is associated with significant diarrhoea, pain and rectal bleeding. Matrix metalloproteinases (MMPs) have been reported to be involved in chemotherapy-induced gut toxicity and RIGT following single-dose irradiation in vivo. We therefore proposed MMPs would be involved in the pathobiology of RIGT following fractionated irradiation.

methodsDark Agouti rats were treated with fractionated radiation (3 × 2.5 Gy/week for 6 weeks). Rats were killed at 3, 6 and 15 weeks to represent acute and chronic toxicities. Sections of jejunum and colon were immunostained for MMP-1, MMP-2, MMP-9 and MMP-14. Relative mRNA expression in jejunum and colon was quantified by RT-PCR for MMP-1, MMP-2, MMP-9 and MMP-14. Western blotting was also conducted on jejunum and colon tissue collected at week 6 to determine protein levels of pro- and active MMP-2.

resultsMMP-2 total protein levels, determined by western blotting, significantly increased in both the jejunum (p = 0.0359) and the colon (p = 0.0134) 6 weeks into the fractionated radiation schedule. MMP-1, MMP-2, and MMP-14 mRNA expression significantly increased in the jejunum. MMP-2 mRNA expression was also significantly increased in the colon. Immunostaining of MMP-2 was observed to be increased in both crypt enterocytes and the lamina propria.

conclusionsMMP-2 plays a role in the pathobiology of gastrointestinal toxicities following fractionated irradiation. Whilst MMP-1 and MMP-14 mRNA expression was increased, this occurred only in the jejunum, suggesting MMPs are differentially involved in RIGT depending on the intestinal region. Further studies are needed to elucidate the role these mediators play in the development and potentiation of RIGT.

Indexed as

AnimalsDose Fractionation, RadiationDose-Response Relationship, RadiationFemaleGastrointestinal DiseasesGene Expression Regulation, EnzymologicIntestinal MucosaIntestine, LargeIntestine, SmallMatrix MetalloproteinasesRadiation DosageRadiation InjuriesRatsRats, TransgenicMatrix MetalloproteinasesEndotheliumGastrointestinalMatrix metalloproteinaseMucositisRadiotherapy

Identifiers

PMID29754212
OpenAlexW2802632798

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.