ArticleApplied microbiology and biotechnology2018
Molecular cloning, expression, and characterization of acyltransferase from Pseudomonas protegens.
Article in Applied microbiology and biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Computational Study of the Fries Rearrangement Catalyzed by Acyltransferase from Pseudomonas protegens.ChemistryOpen · 2024Article
- Characterization and Assessment of 2, 4-Diacetylphloroglucinol (DAPG)-ProducingMicroorganisms · 2022Article
- Phloroglucinol Derivatives in Plant-BeneficialMetabolites · 2021Review
- Oak-Associated Negativicute Equipped with Ancestral Aromatic Polyketide Synthase Produces Antimycobacterial Dendrubins.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Mechanism of Biocatalytic Friedel-Crafts Acylation by Acyltransferase fromACS catalysis · 2020Article
- Thioesters as Acyl Donors in Biocatalytic Friedel-Crafts-type Acylation Catalyzed by Acyltransferase fromChemCatChem · 2019Article
- Clearing the Noise: Seasonal Dynamics of Endophytic Bacteria in Fagus sylvatica Leaves Revealed by Application of PNA Clamps.Physiologia plantarumArticle
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Authors and funding
6 authors.
Funding
Abstract
The formation of C-C bonds by using CoA independent acyltransferases may have significant impact for novel methods for biotechnology. We report the identification of Pseudomonas strains with CoA-independent acyltransferase activity as well as the heterologous expression of the enzyme in E. coli. The cloning strategies and selected expression studies are discussed. The recombinant acyltransferases were characterized with regard to thermal and storage stability, pH,- and co-solvent tolerance. Moreover, the impact of bivalent metals, inhibitors, and other additives was tested. Careful selection of expression and working conditions led to obtain recombinant acyltransferase form Pseudomonas protegens with up to 11 U mL
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