ArticleMicrobial cell factories2018
Biotransformation of steroids by entomopathogenic strains of Isaria farinosa.
Article in Microbial cell factories, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- Antimicrobial Properties of Flavonoid Derivatives with Bromine, Chlorine, and Nitro Group Obtained by Chemical Synthesis and Biotransformation Studies.International journal of molecular sciences · 2024Article
- Multienzymatic biotransformation of flavokawain B by entomopathogenic filamentous fungi: structural modifications and pharmacological predictions.Microbial cell factories · 2024Article
- Biotransformation of ΔInternational journal of molecular sciences · 2023Article
- Identification of a Gene Encoding a New Fungal Steroid 7-Hydroxylase and Its Functional Characterization inInternational journal of molecular sciences · 2023Article
- Glycosylation of Quercetin by Selected Entomopathogenic Filamentous Fungi and Prediction of Its Products' Bioactivity.International journal of molecular sciences · 2023Article
- Biotransformation of Flavonoids with -NOInternational journal of molecular sciences · 2023Article
- Multi-Level Optimization and Strategies in Microbial Biotransformation of Nature Products.Molecules (Basel, Switzerland) · 2023Review
- Hydroxylation of Progesterone and Its Derivatives by the Entomopathogenic StrainInternational journal of molecular sciences · 2022Article
- Hydroxylation of pregnenolone and dehydroepiandrosterone by zygomycete Backusella lamprospora VKM F-944: selective production of 7α-OH-DHEA.Applied microbiology and biotechnology · 2022Article
- Betamethasone Dipropionate Derivatization, Biotransformation, Molecular Docking, and ADME Analysis as Glucocorticoid Receptor.BioMed research international · 2022Article
- Effects of epiphytic and exogenous lactic acid bacteria on fermentation quality and microbial community compositions of paper mulberry silage.Frontiers in microbiology · 2022Article
- Cyclodepsipeptide Biosynthesis inPathogens (Basel, Switzerland) · 2020Article
- New 6,19-oxidoandrostan derivatives obtained by biotransformation in environmental filamentous fungi cultures.Microbial cell factories · 2020Article
- Distinct Regioselectivity of Fungal P450 Enzymes for Steroidal Hydroxylation.Applied and environmental microbiology · 2019Article
- Cascade biotransformation of estrogens by Isaria fumosorosea KCh J2.Scientific reports · 2019Article
- Article
- Secondary Metabolites and the Risks ofMolecules (Basel, Switzerland) · 2019Review
- Culture conditions and nutrition requirements for the mycelial growth of Isaria farinosa (Hypocreales: Cordycipitaceae) and the altitude effect on its growth and metabolome.Scientific reports · 2018Article
- Glycosylation of 3-Hydroxyflavone, 3-Methoxyflavone, Quercetin and Baicalein in Fungal Cultures of the GenusMolecules (Basel, Switzerland) · 2018Article
- Cascade biotransformation of dehydroepiandrosterone (DHEA) by Beauveria species.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSteroid compounds are very interesting substrates for biotransformation due to their high biological activity and a high number of inactivated carbons which make chemical modification difficult. Microbial transformation can involve reactions which are complicated and uneconomical in chemical synthesis, and searching for a new effective biocatalyst is necessary. The best known entomopathogenic species used in steroid modification is Beauveria bassiana. In this study we tested the ability of Isaria farinosa, another entomopathogenic species, to transform several steroids.
resultsTwelve strains of the entomopathogenic filamentous fungus Isaria farinosa, collected in abandoned mines located in the area of the Lower Silesian Voivodeship, Poland, from insects' bodies covered by fungus, were used as a biocatalyst. All the tested strains effectively transformed dehydroepiandrosterone (DHEA). We observed 7α- and 7β-hydroxy derivatives as well as changes in the percentage composition of the emerging products. Due to the similar metabolism of DHEA in all tested strains, one of them was selected for further investigation. In the culture of the selected strain, Isaria farinosa KCh KW1.1, transformations of androstenediol, androstenedione, adrenosterone, 17α-methyltestosterone, 17β-hydroxyandrost-1,4,6-triene-3-one and progesterone were performed. All the substrates were hydroxylated with high yield and stereoselectivity. We obtained 6β-hydroxyandrost-4-ene-3,11,17-trione, 15α,17β-dihydroxy-6β,7β-epoxyandrost-1,4-diene-3-one and 6β,11α-dihydroxyprogesterone. There is no evidence of either earlier microbial transformation of 17β-hydroxyandrost-1,4,6-triene-3-one or new epoxy derivatives.
conclusionsIsaria farinosa has a broad spectrum of highly effective steroid hydroxylases. The obtained 7-hydroxydehydroepiandrosterone has proven high biological activity and can be used in Alzheimer's disease and as a key intermediate in the synthesis of aldosterone antagonists. Transformation of progesterone leads to high yield of 6β,11α-dihydroxyprogesterone and it is worth further study.
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