ArticleComparative biochemistry and physiology. Part A, Molecular & integrative physiology2018
Localization and expression of putative circadian clock transcripts in the brain of the nudibranch Melibe leonina.
Article in Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed, 10 citations in OpenAlex.
- Neural mechanism of circadian clock-based photoperiodism in insects and snails.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2024Review
- In the sea slugFrontiers in physiology · 2022Article
- Diversity of Light Sensing Molecules and Their Expression During the Embryogenesis of the Cuttlefish (Frontiers in physiology · 2020Article
- The Distribution and Possible Roles of Small Cardioactive Peptide in the NudibranchIntegrative organismal biology (Oxford, England) · 2020Article
- Differential Impacts of the Head onFrontiers in physiology · 2019Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
The nudibranch, Melibe leonina, expresses a circadian rhythm of locomotion, and we recently determined the sequences of multiple circadian clock transcripts that may play a role in controlling these daily patterns of behavior. In this study, we used these genomic data to help us: 1) identify putative clock neurons using fluorescent in situ hybridization (FISH); and 2) determine if there is a daily rhythm of expression of clock transcripts in the M. leonina brain, using quantitative PCR. FISH indicated the presence of the clock-related transcripts clock, period, and photoreceptive and non-photoreceptive cryptochrome (pcry and npcry, respectively) in two bilateral neurons in each cerebropleural ganglion and a group of <10 neurons in the anterolateral region of each pedal ganglion. Double-label experiments confirmed colocalization of all four clock transcripts with each other. Quantitative PCR demonstrated that the genes clock, period, pcry and npcry exhibited significant differences in expression levels over 24 h. These data suggest that the putative circadian clock network in M. leonina consists of a small number of identifiable neurons that express circadian genes with a daily rhythm.
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