ArticleApplied and environmental microbiology2018
An Iterative, Synthetic Approach To Engineer a High-Performance PhoB-Specific Reporter.
Article in Applied and environmental microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Corrected and republished from: "Applied and environmental microbiology · 2024Article
- Bacterial growth dynamics in a rhythmic symbiosis.Molecular biology of the cell · 2024Article
- Lighting the way: how theJournal of bacteriology · 2024Review
- Vibrio fischeri Possesses Xds and Dns Nucleases That Differentially Influence Phosphate Scavenging, Aggregation, Competence, and Symbiotic Colonization of Squid.Applied and environmental microbiology · 2022Article
- A lasting symbiosis: how Vibrio fischeri finds a squid partner and persists within its natural host.Nature reviews. Microbiology · 2021Review
- Implication of the Type IV Secretion System in the Pathogenicity ofFrontiers in cellular and infection microbiology · 2021Article
- Sulfur availability for Vibrio fischeri growth during symbiosis establishment depends on biogeography within the squid light organ.Molecular microbiology · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
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Abstract
Transcriptional reporters are common tools for analyzing either the transcription of a gene of interest or the activity of a specific transcriptional regulator. Unfortunately, the latter application has the shortcoming that native promoters did not evolve as optimal readouts for the activity of a particular regulator. We sought to synthesize an optimized transcriptional reporter for assessing PhoB activity, aiming for maximal "on" expression when PhoB is active, minimal background in the "off" state, and no control elements for other regulators. We designed specific sequences for promoter elements with appropriately spaced PhoB-binding sites, and at 19 additional intervening nucleotide positions for which we did not predict sequence-specific effects, the bases were randomized. Eighty-three such constructs were screened in
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Registered trials
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