ArticlePloS one2018
Engineering bacterial motility towards hydrogen-peroxide.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 43 citations in OpenAlex.
- Systems engineering of engineered live biotherapeutics: A discovery-to-translation framework for streamlining microbiome therapeutic development.Journal of controlled release : official journal of the Controlled Release Society · 2026Review
- Engineered E. coli swarming for binary and analog input recording.Molecular systems biology · 2026Article
- Redox active plant phenolic, acetosyringone, for electrogenetic signaling.Scientific reports · 2024Article
- Assessing electrogenetic activation via a network model of biological signal propagation.Frontiers in systems biology · 2024Article
- Redox-enabled electronic interrogation and feedback control of hierarchical and networked biological systems.Nature communications · 2023Article
- Incoherent merger network for robust ratiometric gene expression response.Nucleic acids research · 2023Article
- Bacterial chemotaxis in static gradients quantified in a biopolymer membrane-integrated microfluidic platform.Lab on a chip · 2022Article
- Machine learning-based inverse design for electrochemically controlled microscopic gradients of OProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Electrogenetic Signal Transmission and Propagation in Coculture to Guide Production of a Small Molecule, Tyrosine.ACS synthetic biology · 2022Article
- Achieving spatially precise diagnosis and therapy in the mammalian gut using synthetic microbial gene circuits.Frontiers in bioengineering and biotechnology · 2022Review
- Electronic signals are electrogenetically relayed to control cell growth and co-culture composition.Metabolic engineering communications · 2021Article
- Can thiol-based redox systems be utilized as parts for synthetic biology applications?Redox report : communications in free radical research · 2021Article
- Bioelectronic control of a microbial community using surface-assembled electrogenetic cells to route signals.Nature nanotechnology · 2021Article
- Homologous Quorum Sensing Regulatory Circuit: A Dual-Input Genetic Controller for Modulating Quorum Sensing-Mediated Protein Expression inACS synthetic biology · 2020Article
- Review
- A redox-based electrogenetic CRISPR system to connect with and control biological information networks.Nature communications · 2020Article
- Microfluidic techniques for separation of bacterial cells via taxis.Microbial cell (Graz, Austria) · 2020Review
- Redox Is a Global Biodevice Information Processing Modality.Proceedings of the IEEE. Institute of Electrical and Electronics Engineers · 2019Article
- Plasmid-encoded protein attenuates Escherichia coli swimming velocity and cell growth, not reprogrammed regulatory functions.Biotechnology progress · 2019Article
- Bacterial toxicity of biomimetic green zinc oxide nanoantibiotic: insights into ZnONP uptake and nanocolloid-bacteria interface.Toxicology research · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Synthetic biologists construct innovative genetic/biological systems to treat environmental, energy, and health problems. Many systems employ rewired cells for non-native product synthesis, while a few have employed the rewired cells as 'smart' devices with programmable function. Building on the latter, we developed a genetic construct to control and direct bacterial motility towards hydrogen peroxide, one of the body's immune response signaling molecules. A motivation for this work is the creation of cells that can target and autonomously treat disease, the latter signaled by hydrogen peroxide release. Bacteria naturally move towards a variety of molecular cues (e.g., nutrients) in the process of chemotaxis. In this work, we engineered bacteria to recognize and move towards hydrogen peroxide, a non-native chemoattractant and potential toxin. Our system exploits oxyRS, the native oxidative stress regulon of E. coli. We first demonstrated H2O2-mediated upregulation motility regulator, CheZ. Using transwell assays, we showed a two-fold increase in net motility towards H2O2. Then, using a 2D cell tracking system, we quantified bacterial motility descriptors including velocity, % running (of tumble/run motions), and a dynamic net directionality towards the molecular cue. In CheZ mutants, we found that increased H2O2 concentration (0-200 μM) and induction time resulted in increased running speeds, ultimately reaching the native E. coli wild-type speed of ~22 μm/s with a ~45-65% ratio of running to tumbling. Finally, using a microfluidic device with stable H2O2 gradients, we characterized responses and the potential for "programmed" directionality towards H2O2 in quiescent fluids. Overall, the synthetic biology framework and tracking analysis in this work will provide a framework for investigating controlled motility of E. coli and other 'smart' probiotics for signal-directed treatment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.