ArticleMolecular and cellular biology1988
Concentration dependence of transcriptional transactivation in inducible E1A-containing human cells.
Article in Molecular and cellular biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Oncogenicity of human papillomavirus- or adenovirus-transformed cells correlates with resistance to lysis by natural killer cells.Journal of virology · 1995Article
- Endogenous expression of E1A in human cells enhances the effect of adenovirus E3 on class I major histocompatibility complex antigen expression.Journal of virology · 1993Article
- The adenovirus E1A transforming protein activates the proliferating cell nuclear antigen promoter via an activating transcription factor site.Journal of virology · 1991Article
- Analysis of the specificity and mechanism of transcriptional activation of the human hsp70 gene during infection by DNA viruses.Journal of virology · 1991Article
- Antioncogenic effect of adenovirus E1A in human tumor cells.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Interleukin 6 enhances a cellular activity that functionally substitutes for E1A protein in transactivation.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Expression and interactions of human adenovirus oncoproteins.The Biochemical journal · 1991Review
- Adenovirus E1A protein activates transcription of the E1A gene subsequent to transcription complex formation.Journal of virology · 1991Article
- The use of engineered E1A genes to transactivate the hCMV-MIE promoter in permanent CHO cell lines.Nucleic acids research · 1991Article
- Adenovirus E1A and E1B genes are regulated posttranscriptionally in human lymphoid cells.Journal of virology · 1990Article
- The dose dependence of glucocorticoid-inducible gene expression results from changes in the number of transcriptionally active templates.The EMBO journal · 1990Article
- E1a transactivation of human HSP70 gene promoter substitution mutants is independent of the composition of upstream and TATA elements.Molecular and cellular biology · 1990Article
- Fusion of adenovirus E1A to the glucocorticoid receptor by high-resolution deletion cloning creates a hormonally inducible viral transactivator.Molecular and cellular biology · 1989Article
- Multiple transcription factor binding sites mediate adenovirus E1A transactivation.Journal of virology · 1989Article
- Adeno-associated virus P5 promoter contains an adenovirus E1A-inducible element and a binding site for the major late transcription factor.Journal of virology · 1989Article
- E1a transactivation of the human HSP70 promoter is mediated through the basal transcriptional complex.Molecular and cellular biology · 1989Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The adenovirus E1A proteins are essential for the normal temporal activation of transcription from every other adenoviral early promoter. High-level E1A expression in the absence of viral infection would facilitate biochemical studies of E1A-mediated transactivation. Toward this end, we introduced the adenovirus type 2 E1A gene under the control of the murine mammary tumor virus promoter into HeLa cells. Uninduced cells expressed little or no detectable E1A mRNA. Upon induction, mRNA levels accumulated to about 50% of the level observed in 293 cells. The level of E1A expression in these cells could be controlled by varying the concentration of the inducing glucocorticoid. Under these conditions of varying E1A concentrations, it was observed that activation of the E2, E3, and E4 promoters of H5dl312 initiated at the same E1A concentration and that transcription from each promoter increased as the E1A concentration increased. These results indicate that E1A-mediated transactivation is proportional to the concentration of E1A protein. E1A-dependent transcriptional stimulation of the E4 promoter was reproduced in an in vitro transcription system, demonstrating that expression of only the E1A proteins was sufficient to increase the transcriptional activity of nuclear extracts.
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