ArticleNature protocols2018
High-yield in vitro recordings from neurons functionally characterized in vivo.
Article in Nature protocols, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Early Multimodal Motor Training After Stroke Promotes Motor Recovery and Whole-Brain Structural Remodeling.Journal of the American Heart Association · 2026Article
- Protocol for longitudinal two-photon calcium imaging and holographic optogenetic manipulation to investigate memory in mice.STAR protocols · 2026Article
- A hybrid compass mechanism combining radical pairs and magnetite crystals.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- A primary sensory cortical interareal feedforward inhibitory circuit for tacto-visual integration.Nature communications · 2024Article
- DeepD3, an open framework for automated quantification of dendritic spines.PLoS computational biology · 2024Article
- Functional and structural features of L2/3 pyramidal cells continuously covary with pial depth in mouse visual cortex.Cerebral cortex (New York, N.Y. : 1991) · 2023Article
- In vivo extracellular recordings of thalamic and cortical visual responses reveal V1 connectivity rules.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Preparation of acute midbrain slices containing the superior colliculus and periaqueductal Gray for patch-clamp recordings.PloS one · 2022Article
- An inhalation anaesthesia approach for neonatal mice allowing streamlined stereotactic injection in the brain.Journal of neuroscience methods · 2020Article
- Inducing Different Neuronal Subtypes from Astrocytes in the Injured Mouse Cerebral Cortex.Neuron · 2019Article
- Bioinspired neuron-like electronics.Nature materials · 2019Article
- Optically Induced Calcium-Dependent Gene Activation and Labeling of Active Neurons Using CaMPARI and Cal-Light.Frontiers in synaptic neuroscience · 2019Article
- Multiple Two-Photon Targeted Whole-Cell Patch-Clamp Recordings From Monosynaptically Connected NeuronsFrontiers in synaptic neuroscience · 2019Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vivo two-photon calcium imaging provides detailed information about the activity and response properties of individual neurons. However, in vitro methods are often required to study the underlying neuronal connectivity and physiology at the cellular and synaptic levels at high resolution. This protocol provides a fast and reliable workflow for combining the two approaches by characterizing the response properties of individual neurons in mice in vivo using genetically encoded calcium indicators (GECIs), followed by retrieval of the same neurons in brain slices for further analysis in vitro (e.g., circuit mapping). In this approach, a reference frame is provided by fluorescent-bead tracks and sparsely transduced neurons expressing a structural marker in order to re-identify the same neurons. The use of GECIs provides a substantial advancement over previous approaches by allowing for repeated in vivo imaging. This opens the possibility of directly correlating experience-dependent changes in neuronal activity and feature selectivity with changes in neuronal connectivity and physiology. This protocol requires expertise both in in vivo two-photon calcium imaging and in vitro electrophysiology. It takes 3 weeks or more to complete, depending on the time allotted for repeated in vivo imaging of neuronal activity.
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Registered trials
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