ArticleScientific reports2018
Live-cell imaging of nuclear-chromosomal dynamics in bovine in vitro fertilised embryos.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- From Static to Dynamic: Fluorescence Imaging Technology Advances Precise Embryo Evaluation.Small methods · 2026Review
- Malonic acid promotes glycolytic activity in cumulus cells and improves the quality of bovine oocytes matured in vitro.The Journal of reproduction and development · 2026Article
- Zinc eluted from glassware is a risk factor for embryo development in human and animal assisted reproduction†.Biology of reproduction · 2025Article
- Animal and vegetal materials of mouse oocytes segregate at first zygotic cleavage: a simple mechanism that makes the two-cell blastomeres differ reciprocally from the start.Molecular human reproduction · 2025Article
- Extracting and analyzing micronuclei from mouse two-cell embryos fertilized with freeze-dried spermatozoa.Communications biology · 2025Article
- Chromosome Counting at Meiosis and Mitosis of Mouse Oocytes and Embryos Using Super-Resolution Live-Cell Imaging and CRISPR/dCas9-Mediated Live-FISH.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Excluding alanine from minimum essential medium (MEM) nonessential amino acid supplementation of the culture medium facilitates post-fertilization events and early cleavages of bovine oocytes fertilized in vitro.The Journal of reproduction and development · 2024Article
- Machine learning in time-lapse imaging to differentiate embryos from young vs old mice†.Biology of reproduction · 2024Article
- Effect of ovarian stimulation on developmental speed of preimplantation embryo in a mouse model.The Journal of reproduction and development · 2024Article
- Serum-free spontaneously immortalized bovine oviduct epithelial cell conditioned medium promotes the early development of bovine in vitro fertilized embryos.The Journal of reproduction and development · 2024Article
- Insights into embryonic chromosomal instability: mechanisms of DNA elimination during mammalian preimplantation development.Frontiers in cell and developmental biology · 2024Review
- Hatchability evaluation of bovine IVF embryos using OCT-based 3D image analysis.The Journal of reproduction and development · 2023Article
- Pre-Implantation Bovine Embryo Evaluation-From Optics to Omics and Beyond.Animals : an open access journal from MDPI · 2023Review
- Time-lapse monitoring technologies for the selection of bovine in vitro fertilized embryos with high implantation potential.The Journal of reproduction and development · 2023Review
- Asynchronous division at 4-8-cell stage of preimplantation embryos affects live birth through ICM/TE differentiation.Scientific reports · 2022Article
- Molecular contribution to embryonic aneuploidy and karyotypic complexity in initial cleavage divisions of mammalian development.Development (Cambridge, England) · 2022Article
- Sperm migration, selection, survival, and fertilizing ability in the mammalian oviduct†.Biology of reproduction · 2021Review
- Three-dimensional live imaging of bovine embryos by optical coherence tomography.The Journal of reproduction and development · 2021Article
- A New Toolbox in Experimental Embryology-Alternative Model Organisms for Studying Preimplantation Development.Journal of developmental biology · 2021Review
- Three-Dimensional Live Imaging of Bovine Preimplantation Embryos: A New Method for IVF Embryo Evaluation.Frontiers in veterinary science · 2021Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear/chromosomal integrity is an important prerequisite for the assessment of embryo quality in artificial reproductive technology. However, lipid-rich dark cytoplasm in bovine embryos prevents its observation by visible light microscopy. We performed live-cell imaging using confocal laser microscopy that allowed long-term imaging of nuclear/chromosomal dynamics in bovine in vitro fertilised (IVF) embryos. We analysed the relationship between nuclear/chromosomal aberrations and in vitro embryonic development and morphological blastocyst quality. Three-dimensional live-cell imaging of 369 embryos injected with mRNA encoding histone H2B-mCherry and enhanced green fluorescent protein (EGFP)-α-tubulin was performed from single-cell to blastocyst stage for eight days; 17.9% reached the blastocyst stage. Abnormalities in the number of pronuclei (PN), chromosomal segregation, cytokinesis, and blastomere number at first cleavage were observed at frequencies of 48.0%, 30.6%, 8.1%, and 22.2%, respectively, and 13.0%, 6.2%, 3.3%, and 13.4%, respectively, for abnormal embryos developed into blastocysts. A multivariate analysis showed that abnormal chromosome segregation (ACS) and multiple PN correlated with delayed timing and abnormal blastomere number at first cleavage, respectively. In morphologically transferrable blastocysts, 30-40% of embryos underwent ACS and had abnormal PN. Live-cell imaging may be useful for analysing the association between nuclear/chromosomal dynamics and embryonic development in bovine embryos.
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