ArticleCell death & disease2018
MiR-455-3p inhibits the degenerate process of chondrogenic differentiation through modification of DNA methylation.
Article in Cell death & disease, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- The microRNA-455 null mouse shows dysregulated bone turnover.JBMR plus · 2025Article
- Molecular Determinants of Neutrophil Extracellular Vesicles That Drive Cartilage Regeneration in Inflammatory Arthritis.Arthritis & rheumatology (Hoboken, N.J.) · 2024Article
- CD34+ synovial fibroblasts exhibit high osteogenic potential in synovial chondromatosis.Cell and tissue research · 2024Article
- MiR-29a-3p mediates phosphatase and tensin homolog and inhibits osteoarthritis progression.Functional & integrative genomics · 2024Article
- Comparison of genome-wide DNA methylation patterns between antler precartilage and cartilage.Molecular genetics and genomics : MGG · 2023Article
- CircNFIX regulates chondrogenesis and cartilage homeostasis by targeting the miR758-3p/KDM6A axis.Cell proliferation · 2022Article
- Global DNA methylation and chondrogenesis of rat limb buds in a three-dimensional organ culture system.Bosnian journal of basic medical sciences · 2022Article
- Cartilage Homeostasis and Osteoarthritis.International journal of molecular sciences · 2022Review
- Mouse microRNA signatures in joint ageing and post-traumatic osteoarthritis.Osteoarthritis and cartilage open · 2021Article
- Integrated analysis of miRNA and mRNA transcriptomic reveals antler growth regulatory network.Molecular genetics and genomics : MGG · 2021Article
- Long Non-coding RNAs LOC100126784 and POM121L9P Derived From Bone Marrow Mesenchymal Stem Cells Enhance Osteogenic Differentiation via the miR-503-5p/SORBS1 Axis.Frontiers in cell and developmental biology · 2021Article
- miR-103a-3p and miR-22-5p Are Reliable Reference Genes in Extracellular Vesicles From Cartilage, Adipose Tissue, and Bone Marrow Cells.Frontiers in bioengineering and biotechnology · 2021Article
- The Synovium Attenuates Cartilage Degeneration in KOA through Activation of the Smad2/3-Runx1 Cascade and Chondrogenesis-related miRNAs.Molecular therapy. Nucleic acids · 2020Article
- Exosomal miRNAs in osteoarthritis.Molecular biology reports · 2020Review
- Interplay between genetics and epigenetics in osteoarthritis.Nature reviews. Rheumatology · 2020Review
- DNA methylation of noncoding RNAs: new insights into osteogenesis and common bone diseases.Stem cell research & therapy · 2020Review
- Inhibition of miR-490-5p Promotes Human Adipose-Derived Stem Cells Chondrogenesis and Protects Chondrocytes via the PITPNM1/PI3K/AKT Axis.Frontiers in cell and developmental biology · 2020Article
- Substantive molecular and histological changes within the meniscus with tears.BMC musculoskeletal disorders · 2019Article
- Non-Coding RNAs in Cartilage Development: An Updated Review.International journal of molecular sciences · 2019Review
- Long Non-coding RNA HOTTIP Promotes CCL3 Expression and Induces Cartilage Degradation by Sponging miR-455-3p.Frontiers in cell and developmental biology · 2019Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this work was to determine whether miR-455-3p regulates DNA methylation during chondrogenic differentiation of hMSCs. The expression of miR-455-3p and de novo methyltransferase DNMT3A was assessed in micromass culture of hBMSCs, which induced chondrogenic differentiation in vitro, and in E16.5 mice in vivo. A luciferase reporter assay was used to confirm whether miR-455-3p directly targets DNMT3A by interaction with the 3'-UTR. Using an Illumina Infinium Methylation EPIC microarray, genome-wide DNA methylation of hBMSCs with or without overexpressed miR-455-3p was examined for 28 days during induced chondrogenic differentiation. Here, we showed that miR-455-3p was more expressed during the middle stage of hBMSC chondrogenic differentiation, and less expressed in the late stage. DNMT3A was less expressed in the middle stage and more expressed in the late stage, and was also more expressed in the palms of miR-455-3p deletion mice compared to those of wild-type mice. The luciferase reporter assay demonstrated that miR-455-3p directly targets DNMT3A 3'-UTR. miR-455-3p overexpression inhibits the degenerate process during chondrogenic differentiation, while deletion of miR-455-3p in mice accelerated cartilage degeneration. Genome-wide DNA methylation analysis showed miR-455-3p overexpression regulates DNA methylation of cartilage-specific genes. GO analysis revealed PI3K-Akt signaling pathway was most hypomethylated. Our data show that miR-455-3p can regulate hMSC chondrogenic differentiation by affecting DNA methylation. Overexpression of miR-455-3p and DNA methylation inhibitors can thus potentially be utilized to optimize chondrogenic differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.