ArticleScientific reports2018
Exploring DNA quality of single cells for genome analysis with simultaneous whole-genome amplification.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 36 citations in OpenAlex.
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- Cell lineage analysis with somatic mutations reveals late divergence of neuronal cell types and cortical areas in human cerebral cortex.bioRxiv : the preprint server for biology · 2023Article
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- Distinct HIV-1 Population Structure across Meningeal and Peripheral T Cells and Macrophage Lineage Cells.Microbiology spectrum · 2022Article
- Determining the Minimally Effective Dose of a Clinical Candidate Adeno-Associated Virus Vector in a Mouse Model of Hemophilia A.Human gene therapy · 2022Article
- Somatic Single-Nucleotide Variant Calling from Single-Cell DNA Sequencing Data Using SCAN-SNV.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Accurate and scalable variant calling from single cell DNA sequencing data with ProSolo.Nature communications · 2021Article
- Dissecting single-cell genomes through the clonal organoid technique.Experimental & molecular medicine · 2021Review
- The Application of Single-Cell RNA Sequencing in Studies of Autoimmune Diseases: a Comprehensive Review.Clinical reviews in allergy & immunology · 2021Review
- Methods for copy number aberration detection from single-cell DNA-sequencing data.Genome biology · 2020Review
- Eleven grand challenges in single-cell data science.Genome biology · 2020Review
- Article
- Whole genome amplification of single epithelial cells dissociated from snap-frozen tissue samples in microfluidic platform.Biomicrofluidics · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single cell genome analysis methods are powerful tools to define features of single cells and to identify differences between them. Since the DNA amount of a single cell is very limited, cellular DNA usually needs to be amplified by whole-genome amplification before being subjected to further analysis. A single nucleus only contains two haploid genomes. Thus, any DNA damage that prevents amplification results in loss of damaged DNA sites and induces an amplification bias. Therefore, the assessment of single cell DNA quality is urgently required. As of today, there is no simple method to determine the quality of a single cell DNA in a manner that will still retain the entire cellular DNA for amplification and downstream analysis. Here, we describe a method for whole-genome amplification with simultaneous quality control of single cell DNA by using a competitive spike-in DNA template.
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