Evidence map›Paper›PMID 29747121›Full record

ArticleVirology2018

Generation of a novel next-generation sequencing-based method for the isolation of new human papillomavirus types.

Rosario N Brancaccio, Alexis Robitaille, Sankhadeep Dutta, Cyrille Cuenin, Daiga Santare, Girts Skenders, Marcis Leja, Nicole Fischer, Anna R Giuliano, Dana E Rollison and 3 more

Abstract read
In one paragraph

Article in Virology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed.

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  18. Isolation of a Novel Beta-2 Human Papillomavirus from Skin.Microbiology resource announcements · 2019
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Rosario N BrancaccioInternational Agency for Research on Cancer, Lyon, France.
Alexis RobitailleInternational Agency for Research on Cancer, Lyon, France.
Sankhadeep DuttaInternational Agency for Research on Cancer, Lyon, France.
Cyrille CueninInternational Agency for Research on Cancer, Lyon, France.
Daiga SantareInstitute of Clinical and Preventive Medicine, University of Latvia, Riga, Latvia.
Girts SkendersInstitute of Clinical and Preventive Medicine, University of Latvia, Riga, Latvia.
Marcis LejaInstitute of Clinical and Preventive Medicine, University of Latvia, Riga, Latvia.
Nicole FischerInstitute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; German Center for Infection Research, partner site Hamburg, Borstel, Lübeck, Riems, Germany.
Anna R GiulianoCenter for Infection Research in Cancer, Moffitt Cancer Center, Tampa, FL, USA.
Dana E RollisonCenter for Infection Research in Cancer, Moffitt Cancer Center, Tampa, FL, USA; Department of Cancer Epidemiology, Moffitt Cancer Center, Tampa, FL, USA.
Adam GrundhoffGerman Center for Infection Research, partner site Hamburg, Borstel, Lübeck, Riems, Germany; Heinrich-Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
Massimo TommasinoInternational Agency for Research on Cancer, Lyon, France. Electronic address: icb@iarc.fr.
Tarik GheitInternational Agency for Research on Cancer, Lyon, France. Electronic address: gheitt@iarc.fr.

Funding

Prospective study of cutaneous viral infections and non-melanoma skin cancerR01CA177586 · NCI · H. LEE MOFFITT CANCER CTR & RES INST · PI ROLLISON, DANA ELISE · 2014 to 2018
$3.4M
NCI NIH HHS R01 CA177586World Health Organization 001
6 · The paper itself

Abstract

With the advent of new molecular tools, the discovery of new papillomaviruses (PVs) has accelerated during the past decade, enabling the expansion of knowledge about the viral populations that inhabit the human body. Human PVs (HPVs) are etiologically linked to benign or malignant lesions of the skin and mucosa. The detection of HPV types can vary widely, depending mainly on the methodology and the quality of the biological sample. Next-generation sequencing is one of the most powerful tools, enabling the discovery of novel viruses in a wide range of biological material. Here, we report a novel protocol for the detection of known and unknown HPV types in human skin and oral gargle samples using improved PCR protocols combined with next-generation sequencing. We identified 105 putative new PV types in addition to 296 known types, thus providing important information about the viral distribution in the oral cavity and skin.

Indexed as

Cohort StudiesDNA PrimersDNA, ViralGenotypeHigh-Throughput Nucleotide SequencingHumansMouthPapillomaviridaePapillomavirus InfectionsPolymerase Chain ReactionProspective StudiesSequence Analysis, DNASkinDNA PrimersDNA, ViralBroad-spectrum HPV PCR primersNew papillomavirusesNext-generation sequencing

Identifiers

PMID29747121
PMCPMC9280450

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.