ArticleConnective tissue research2018
Crosstalk between neuropeptides SP and CGRP in regulation of BMP2-induced bone differentiation.
Article in Connective tissue research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
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- Feasibility of administration of calcitonin gene-related peptide receptor antagonist on attenuation of pain and progression in osteoarthritis.Scientific reports · 2023Article
- Research Progress in Calcitonin Gene-Related Peptide and Bone Repair.Biomolecules · 2023Review
- The Cell-Specific Role of SHP2 in Regulating Bone Homeostasis and Regeneration Niches.International journal of molecular sciences · 2023Review
- Function of peripheral nerves in the development and healing of tendon and bone.Seminars in cell & developmental biology · 2022Review
- Review
- Exploring the effect of the "quaternary regulation" theory of "peripheral nerve-angiogenesis-osteoclast-osteogenesis" on osteoporosis based on neuropeptides.Frontiers in endocrinology · 2022Review
- Cell trafficking and regulation of osteoblastogenesis by extracellular vesicle associated bone morphogenetic protein 2.Journal of extracellular vesicles · 2021Article
- Schwann cells promote prevascularization and osteogenesis of tissue-engineered bone via bone marrow mesenchymal stem cell-derived endothelial cells.Stem cell research & therapy · 2021Article
- Crosstalk between Bone and Nerves within Bone.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- CGRP Regulates the Age-Related Switch Between Osteoblast and Adipocyte Differentiation.Frontiers in cell and developmental biology · 2021Article
- Neuromodulation of bone: Role of different peptides and their interactions (Review).Molecular medicine reports · 2021Review
- Deficiency of Calcitonin Gene-Related Peptide Affects Macrophage Polarization in Osseointegration.Frontiers in physiology · 2020Article
- A novel hybrid compound LLP2A-alendronate accelerates open fracture healing in a rabbit model.Drug design, development and therapy · 2019Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
aim of the studyThe peripheral nervous system is involved in regulation of bone metabolism via sensory and sympathetic innervation. Substance P (SP) and calcitonin gene-related peptide (CGRP) are two sensory neuropeptides that have been associated with regulation of osteogenic differentiation. However, the interaction between SP and CGRP both with each other and the bone morphogenetic protein 2 (BMP2) in regulation of osteogenic differentiation has not been studied. Therefore, the aim of this study was to investigate the interaction between SP and CGRP on BMP2-induced bone differentiation using model progenitor cells. MATERIALS AND
methodsC2C12 myoblasts and MC3T3 pre-osteoblasts were treated with SP and CGRP, both individually and in combination, in the presence of BMP2. The effects of the neuropeptides on BMP2-induced osteogenic differentiation were assessed by measuring alkaline phosphatase (ALP) activity, mineralization, and expression of osteogenic markers.
resultsBoth SP and CGRP enhanced BMP2 signaling, Runx2 mRNA expression, as well as mineralization in vitro. Co-stimulation with SP and CGRP resulted in down-regulation of BMP2-induced bone differentiation, suggesting potential crosstalk between the two neuropeptides in regulation of BMP2 signaling.
conclusionsBased on the results shown here, CGRP can mitigate augmenting effects of SP on BMP2 signaling and the three pathways potentially converge on Runx2 to regulate BMP2-induced bone differentiation.
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