ArticleThe World Allergy Organization journal2018
Extended IgE profile based on an allergen macroarray: a novel tool for precision medicine in allergy diagnosis.
Article in The World Allergy Organization journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 111 citations in OpenAlex.
- Seasonality of Serum Allergen-Specific IgE Levels in Scandinavian Dogs Suspected of Allergy.Veterinary sciences · 2026Article
- Molecular Biomarkers in IgE Immunoassays Used for Grass Pollen Allergy Diagnosis in European Clinical Settings.International journal of molecular sciences · 2026Review
- Molecular sensitization clusters in adult atopy: an ALEXFrontiers in immunology · 2026Article
- Socioenvironmental factors associated with shrimp allergy and shrimp sensitization in a large and diverse patient population and cohort study from metropolitan Detroit.The journal of allergy and clinical immunology. Global · 2025Article
- HLA variation associated with peanut allergy and anaphylaxis among non-Hispanic Black individuals.The journal of allergy and clinical immunology. Global · 2025Article
- Component-Resolved and Multiplex-Specific IgE Diagnostics: Utility in Anaphylaxis and Beyond.Children (Basel, Switzerland) · 2025Review
- Big Data-Driven Evolution of a Diagnostic Multiplex IgE-Test: Enhancing Accuracy and Efficacy in Allergy Diagnostics.International journal of molecular sciences · 2025Article
- Article
- A Comparison of asIgE Levels Measured with ALEX and ImmunoCAP ISAC in Polish Children with Food Allergies.International journal of molecular sciences · 2025Article
- IgE sensitization to house dust mite and cockroach allergens in asthmatic and allergic patients in the tropics.Frontiers in allergy · 2025Article
- Multiplex IgE peanut panels: a critical appraisal of assay designs and the good, the bad, and the ugly features of the applied allergen components.Frontiers in allergy · 2025Article
- From skin testing to molecular diagnostics: the precision leap in dust mite allergy diagnosis and clinical translation challenges.Frontiers in allergy · 2025Review
- Protein Biochips with Three-Dimensional Hydrogel or Polymer Brush Elements for the Detection of Human Serum Immunoglobulin E Specific to Inhalation Allergens.International journal of molecular sciences · 2024Article
- Molecular allergen sensitization drives phenotypes of severe asthma in children: Evidence from a megacity cohort (SAMP).Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology · 2024Article
- Validation of a Multiplex Molecular Macroarray for the Determination of Allergen-Specific IgE Sensitizations in Dogs.Veterinary sciences · 2024Article
- Allergen Microarrays and New Physical Approaches to More Sensitive and Specific Detection of Allergen-Specific Antibodies.Biosensors · 2024Review
- Single-Domain Antibodies-Novel Tools to Study and Treat Allergies.International journal of molecular sciences · 2024Review
- Multiplex Assays in Allergy Diagnosis: Allergy Explorer 2 versus ImmunoCAP ISAC E112i.Diagnostics (Basel, Switzerland) · 2024Article
- The role of molecular diagnosis in anaphylactic patients with dual or triple-sensitization to Hymenoptera venoms.Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology · 2024Article
- Decreased skin colonization withFrontiers in medicine · 2024Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPrecision medicine (PM) is changing the scope of allergy diagnosis and treatment. An in vitro IgE assay, a prototype PM method, was developed in the sixties and has garnered increasing interest because of the introduction of recombinant components in the test. More recently, microarrays of allergen components have significantly improved the ability to describe the IgE profile. Aim of this study was to evaluate the characteristics of the newly developed Allergy Explorer (ALEX), a macroarray containing both extracted "whole" allergens and molecular components. This method allows the acquisition of an IgE profile comprising 282 reagents (157 allergen extracts and 125 components), resulting in the widest screening of potential allergens available.
methodsSera from 43 patients with allergies were assayed with ALEX and then with ImmunoCAP ISAC. The results of the two tests were compared, and the consistency of the molecular results with the presence of IgE in the relevant extract was also evaluated.
resultsA good correlation between ISAC and ALEX was observed. The ALEX results for second-level tests (i.e., specific IgE to complete extracted allergens) were consistent with the results obtained for the relevant components. DISCUSSION: Despite differences in the methodology, the IgE profiles detected for molecular allergens by ALEX and ISAC were very similar. The differences were mainly related to the lower dynamic range of ALEX and to the use of a CCD inhibitor in the first incubation phase, which reduced the binding of IgE to CCD, as represented in the extracted allergens and components.
conclusionBased on our findings, ALEX is a novel tool for describing the IgE profile in a PM setting, where the IgE assay must be performed on many allergens and components. In particular, polysensitized patients and patients with pollen-food syndrome will have a real advantage due the combination of the second and third levels of allergy diagnostics in the same chip.
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