ArticleScientific reports2018
qSR: a quantitative super-resolution analysis tool reveals the cell-cycle dependent organization of RNA Polymerase I in live human cells.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Histone acetylation-dependent clustering of BRD2 instructs transcription dynamics.Nature genetics · 2026Article
- mTORC1 regulates the pyrimidine salvage pathway by controlling UCK2 turnover via the CTLH-WDR26 E3 ligase.Cell reports · 2025Article
- Human RNA Polymerase II Segregates from Genes and Nascent RNA and Transcribes in the Presence of DNA-Bound dCas9.International journal of molecular sciences · 2024Article
- Developmental Changes in Genome Replication Progression in Pluripotent versus Differentiated Human Cells.Genes · 2024Article
- YTHDF2 facilitates aggresome formation via UPF1 in an mNature communications · 2023Article
- The dynamic clustering of insulin receptor underlies its signaling and is disrupted in insulin resistance.Nature communications · 2022Article
- The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors.Nature communications · 2022Article
- Mapping subcellular localizations of unannotated microproteins and alternative proteins with MicroID.Molecular cell · 2022Article
- Hijacking of transcriptional condensates by endogenous retroviruses.Nature genetics · 2022Article
- CTCF-mediated chromatin looping provides a topological framework for the formation of phase-separated transcriptional condensates.Nucleic acids research · 2022Article
- Fluorescence microscopy imaging of a neurotransmitter receptor and its cell membrane lipid milieu.Frontiers in molecular biosciences · 2022Review
- Physical Nature of Chromatin in the Nucleus.Cold Spring Harbor perspectives in biology · 2021Review
- A dual role for H2A.Z.1 in modulating the dynamics of RNA polymerase II initiation and elongation.Nature structural & molecular biology · 2021Article
- Single-molecule tracking of transcription protein dynamics in living cells: seeing is believing, but what are we seeing?Current opinion in genetics & development · 2021Review
- Article
- Transcriptional suppression of ribosomal DNA with phase separation.Science advances · 2020Article
- Super-resolution in situ analysis of active ribosomal DNA chromatin organization in the nucleolus.Scientific reports · 2020Article
- A first order phase transition mechanism underlies protein aggregation in mammalian cells.eLife · 2019Article
- Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stability.Scientific reports · 2018Article
- Mediator and RNA polymerase II clusters associate in transcription-dependent condensates.Science (New York, N.Y.) · 2018Article
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10 authors.
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Abstract
We present qSR, an analytical tool for the quantitative analysis of single molecule based super-resolution data. The software is created as an open-source platform integrating multiple algorithms for rigorous spatial and temporal characterizations of protein clusters in super-resolution data of living cells. First, we illustrate qSR using a sample live cell data of RNA Polymerase II (Pol II) as an example of highly dynamic sub-diffractive clusters. Then we utilize qSR to investigate the organization and dynamics of endogenous RNA Polymerase I (Pol I) in live human cells, throughout the cell cycle. Our analysis reveals a previously uncharacterized transient clustering of Pol I. Both stable and transient populations of Pol I clusters co-exist in individual living cells, and their relative fraction vary during cell cycle, in a manner correlating with global gene expression. Thus, qSR serves to facilitate the study of protein organization and dynamics with very high spatial and temporal resolutions directly in live cell.
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