ArticleBiophysical journal2018
Single-Molecule Light-Sheet Imaging of Suspended T Cells.
Article in Biophysical journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Light sheet microscopy imaging dataset of CAR-T-cell-mediated cytotoxicity.Scientific data · 2026Article
- Stress-Induced Reorganization of Proteasomes Through Diffusion and Cytoskeleton-Dependent Mechanisms.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- In-depth single molecule localization microscopy using adaptive optics and single objective light-sheet microscopy.Nature communications · 2025Article
- 3D live imaging and phenotyping of CAR-T cell mediated-cytotoxicity using high-throughput Bessel oblique plane microscopy.Nature communications · 2024Article
- Increased vesicular dynamics and nanoscale clustering of IL-2 after T cell activation.Biophysical journal · 2024Article
- Membrane topography and the overestimation of protein clustering in single molecule localisation microscopy - identification and correction.Communications biology · 2024Article
- High-density volumetric super-resolution microscopy.Nature communications · 2024Article
- Optical sensing and control of T cell signaling pathways.Frontiers in physiology · 2023Review
- Surfaces for Study of Receptor Dynamics on T Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Single-molecule and super-resolved imaging deciphers membrane behavior of onco-immunogenic CCR5.iScience · 2022Article
- resPAINT: Accelerating Volumetric Super-Resolution Localisation Microscopy by Active Control of Probe Emission.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2022Article
- resPAINT: Accelerating Volumetric Super-Resolution Localisation Microscopy by Active Control of Probe Emission.Angewandte Chemie (International ed. in English) · 2022Article
- Understanding immune signaling using advanced imaging techniques.Biochemical Society transactions · 2022Review
- 3D Single Molecule Super-Resolution Microscopy of Whole Nuclear Lamina.Frontiers in chemistry · 2022Article
- Rapid statistical discrimination of fluorescence images of T cell receptors on immobilizing surfaces with different coating conditions.Scientific reports · 2021Article
- Lateral diffusion of CD14 and TLR2 in macrophage plasma membrane assessed by raster image correlation spectroscopy and single particle tracking.Scientific reports · 2020Article
- A biophysical perspective on receptor-mediated virus entry with a focus on HIV.Biochimica et biophysica acta. Biomembranes · 2020Review
- MyD88 Death-Domain Oligomerization Determines Myddosome Architecture: Implications for Toll-like Receptor Signaling.Structure (London, England : 1993) · 2020Article
- T Cell Membrane Heterogeneity Aids Antigen Recognition and T Cell Activation.Frontiers in cell and developmental biology · 2020Review
- Calcium Signaling in T Cells Is Induced by Binding to Nickel-Chelating Lipids in Supported Lipid Bilayers.Frontiers in physiology · 2020Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Adaptive immune responses are initiated by triggering of the T cell receptor. Single-molecule imaging based on total internal reflection fluorescence microscopy at coverslip/basal cell interfaces is commonly used to study this process. These experiments have suggested, unexpectedly, that the diffusional behavior and organization of signaling proteins and receptors may be constrained before activation. However, it is unclear to what extent the molecular behavior and cell state is affected by the imaging conditions, i.e., by the presence of a supporting surface. In this study, we implemented single-molecule light-sheet microscopy, which enables single receptors to be directly visualized at any plane in a cell to study protein dynamics and organization in live, resting T cells. The light sheet enabled the acquisition of high-quality single-molecule fluorescence images that were comparable to those of total internal reflection fluorescence microscopy. By comparing the apical and basal surfaces of surface-contacting T cells using single-molecule light-sheet microscopy, we found that most coated-glass surfaces and supported lipid bilayers profoundly affected the diffusion of membrane proteins (T cell receptor and CD45) and that all the surfaces induced calcium influx to various degrees. Our results suggest that, when studying resting T cells, surfaces are best avoided, which we achieve here by suspending cells in agarose.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.