ArticleBiophysical journal2018
Traction Force Screening Enabled by Compliant PDMS Elastomers.
Article in Biophysical journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed.
- Optical Detection of Cellular Signals at Material Interfaces.Advanced healthcare materials · 2026Review
- Stiffening of soft silicone upon deep UV treatment as characterized using nanoindentation.PloS one · 2026Article
- Hierarchical Structure of the Program Used by Filamentous Fungi to Navigate in Confining Microenvironments.Biomimetics (Basel, Switzerland) · 2025Article
- Article
- N-cadherin antagonism is bronchoprotective in severe asthma models.Science advances · 2024Article
- Inferring cellular contractile forces and work using deep morphology traction microscopy.Biophysical journal · 2024Article
- Insights into the mechanobiology of cancer metastasis via microfluidic technologies.APL bioengineering · 2024Review
- Assessing friction and damage of cell monolayers on soft substratesJournal of the Royal Society, Interface · 2024Article
- Nuclear curvature determines Yes-associated protein localization and differentiation of mesenchymal stem cells.Biophysical journal · 2024Article
- Dynamic control of contractile resistance to iPSC-derived micro-heart muscle arrays.Journal of biomedical materials research. Part A · 2024Article
- MAP4K4 regulates forces at cell-cell and cell-matrix adhesions to promote collective cell migration.Life science alliance · 2023Article
- Nuclear compression regulates YAP spatiotemporal fluctuations in living cells.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Multiscale stiffness of human emphysematous precision cut lung slices.Science advances · 2023Article
- Contraction pressure analysis using optical imaging in normal and MYBPC3-mutated hiPSC-derived cardiomyocytes grown on matrices with tunable stiffness.Biomaterials and biosystems · 2022Article
- Migration and 3D Traction Force Measurements inside Compliant Microchannels.Nano letters · 2022Article
- Lysyl oxidase like 2 is increased in asthma and contributes to asthmatic airway remodelling.The European respiratory journal · 2022Article
- A primer to traction force microscopy.The Journal of biological chemistry · 2022Review
- Hydrophobic Recovery of PDMS Surfaces in Contact with Hydrophilic Entities: Relevance to Biomedical Devices.Materials (Basel, Switzerland) · 2022Article
- Cell monolayer deformation microscopy reveals mechanical fragility of cell monolayers following EMT.Biophysical journal · 2022Article
- Prostate cancer cells of increasing metastatic potential exhibit diverse contractile forces, cell stiffness, and motility in a microenvironment stiffness-dependent manner.Frontiers in cell and developmental biology · 2022Article
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
Actomyosin contractility is an essential element of many aspects of cellular biology and manifests as traction forces that cells exert on their surroundings. The central role of these forces makes them a novel principal therapeutic target in diverse diseases. This requires accurate and higher-capacity measurements of traction forces; however, existing methods are largely low throughput, limiting their utility in broader applications. To address this need, we employ Fourier-transform traction force microscopy in a parallelized 96-well format, which we refer to as contractile force screening. Critically, rather than the frequently employed hydrogel polyacrylamide, we fabricate these plates using polydimethylsiloxane rubber. Key to this approach is that the polydimethylsiloxane used is very compliant, with a lower-bound Young's modulus of ∼0.4 kPa. We subdivide these monolithic substrates spatially into biochemically independent wells, creating a uniform multiwell platform for traction force screening. We demonstrate the utility and versatility of this platform by quantifying the compound and dose-dependent contractility responses of human airway smooth muscle cells and retinal pigment epithelial cells. By directly quantifying the endpoint of therapeutic intent, airway-smooth-muscle contractile force, this approach fills an important methodological void in current screening approaches for bronchodilator drug discovery, and, more generally, in measuring contractile response for a broad range of cell types and pathologies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.