ArticleBiodemography and social biology
HPLC-based Measurement of Glycated Hemoglobin using Dried Blood Spots Collected under Adverse Field Conditions.
Article in Biodemography and social biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.
- Dried Blood Spots to Assess Cardiovascular-Kidney-Metabolic Health.Journal of the American Heart Association · 2025Pooled it
- Prevalence, awareness, treatment, and control of diabetes in India: a nationally representative survey of adults aged 45 years and older.The Lancet. Global health · 2025Article
- Undiagnosed diabetes mellitus among older adults: a harmonized cross-country analysis of prevalence, regional disparities and risk factors in 11 European countries and Israel.BMC public health · 2025Article
- First Reported Case of Hemoglobin Graz in the United States: Implications for Misleading Hemoglobin AJCEM case reports · 2025Article
- The link between serum neuregulin-1 and atherogenic index in type 2 diabetes mellitus.Irish journal of medical science · 2024Article
- Explaining adverse cholesterol levels and distinct gender patterns in an Indonesian population compared with the U.S.Economics and human biology · 2024Article
- Clinical and Metabolic Particularities of a Roma Population with Diabetes-Considering Ethnic Disparities in Approaching Healthcare Management.Biomedicines · 2024Article
- Article
- Postal methods for monitoring HbA1c in diabetes mellitus: a protocol for systematic review.BJGP open · 2022Article
- Dried blood spot collection, sample quality, and fieldwork conditions: Structural validations for conversion into standard values.American journal of human biology : the official journal of the Human Biology Council · 2021Article
- Dried blood spots: Effects of less than optimal collection, shipping time, heat, and humidity.American journal of human biology : the official journal of the Human Biology Council · 2020Article
- Design and measurement in a study of war exposure, health, and aging: protocol for the Vietnam health and aging study.BMC public health · 2019Article
Corrections and comments
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Authors and funding
8 authors at 6 institutions in 2 countries.
Funding
Abstract
Glycated hemoglobin (HbA1c) measured using high-performance liquid chromatography (HPLC) assays with venous blood and dried blood spots (DBS) are compared for 143 paired samples collected in Aceh, Indonesia. Relative to gold-standard venous-blood values, DBS-based values reported by the HPLC are systematically upward biased for HbA1c<8% and the fraction diabetic (HbA1c ≥ 6.5%) is overstated almost five-fold. Inspection of chromatograms from DBS assays indicates the % glycosylated calculated by the HPLC excludes part of the hemoglobin A which is misidentified as a hemoglobin variant. Taking this into account, unbiased DBS-based values are computed using data from the machine-generated chromatograms. When the DBS are collected in a clinic-like setting, under controlled humidity/temperature conditions, the recalculated values are almost identical to venous-based values. When DBS are collected under field conditions, the recalculated values are unbiased, but only about half the HbA1c values are measured reliably, calling into question the validity of the other half. The results suggest that collection conditions, particularly humidity, affect the quality of the DBS-based measures. Cross-validating DBS-based HbA1c values with venous samples collected under exactly the same environmental conditions is a prudent investment in population-based studies.
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Registered trials
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