Evidence map›Paper›PMID 29740695›Full record

ArticleEuropean journal of nutrition2019

Metabolic influence of walnut phenolic extract on mitochondria in a colon cancer stem cell model.

Jina Choi, Phil-Kyung Shin, Yuri Kim, Chang Pyo Hong, Sang-Woon Choi

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Article in European journal of nutrition, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jina ChoiCHA University School of Medicine, Seongnam, South Korea.
Phil-Kyung ShinCHA University School of Medicine, Seongnam, South Korea.
Yuri KimNutritional Science and Food Management, Ewha Womans University, Seoul, South Korea.
Chang Pyo HongTeragen Etex, Suwon, South Korea.
Sang-Woon ChoiCHA University School of Medicine, Seongnam, South Korea. sang.choi@cha.ac.kr.ORCID http://orcid.org/0000-0003-0713-379X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeWalnut phenolic extract (WPE) reduces proliferation and enhances differentiation of colon cancer stem cells (CSCs). The present study investigated the metabolic influence of WPE on the mitochondrial function of colon CSCs to determine its underlying mechanism.

methodsCD133

resultsRNA-Seq analysis identified two major functional clusters: metabolic and mitochondrial clusters. WPE treatment shifted the metabolic profile of cells towards the glycolysis pathway (ΔECAR = 36.98 mpH/min/ptn, p = 0.02) and oxidative pathway (ΔOCR = 29.18 pmol/min/ptn, p = 0.00001). Serial mitochondrial stimulations using respiration modulators, oligomycin, carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone, and rotenone/antimycin A, found an increased potential of mitochondrial respiration (ΔOCR = 111.5 pmol/min/ptn, p = 0.0006). WPE treatment also increased glucose uptake (Δ = 0.39 pmol/µL, p = 0.002) and lactate production (Δ = 0.08 nmol/µL, p = 0.005).

conclusionsWPE treatment shifts the mitochondrial metabolism of colon CSC towards more aerobic glycolysis, which might be associated with the alterations in the characteristics of colon CSC.

Indexed as

Cell DifferentiationCell ProliferationCells, CulturedColonic NeoplasmsEnergy MetabolismGlycolysisHumansJuglansMitochondriaNeoplastic Stem CellsOxygen ConsumptionPlant ExtractsPlant ExtractsColon cancer stem cellGlycolysisMetabolic reprogrammingMitochondriaWalnut

Identifiers

PMID29740695

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.