ArticleScientific reports2018
Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 34 citations in OpenAlex.
- Review
- Review
- Fetal and neonatal demise in zoonotic diseases: pathology and pathogenesis.Infection and immunity · 2026Review
- Development of serologic diagnostic test based on in silico predicted synthetic peptides for Brucella canis in dogs.PloS one · 2026Article
- Joint Epidemiological Investigation Results of the Canine Brucellosis Outbreak at a Breeding Facility Associated with a Human Brucellosis Case, 2025.Public health weekly report · 2026Article
- Global epidemiology and diagnostic insights into canine brucellosis: A comprehensive meta-analysis and meta-regression.One health (Amsterdam, Netherlands) · 2025Review
- Tick-borne encephalitis virus associated with foetal death in a bitch, a case report.Virology journal · 2025Article
- Necropsy Technique and Histological Characterisation of Organs From Neonatal Puppies: What Do We Know?Veterinary medicine and science · 2025Article
- "My Bitch Is Empty!" An Overview of the Reasons for Pregnancy Loss in Dogs.Veterinary sciences · 2025Review
- Brucella abortus impairs T lymphocyte responsiveness by mobilizing IL-1RA-secreting omental neutrophils.Nature communications · 2025Article
- In Silico Design and Characterization of a Multiepitope Vaccine Candidate AgainstJournal of immunology research · 2025Article
- Immune Responses Potentially Involved in the Gestational Complications ofPathogens (Basel, Switzerland) · 2023Review
- The emergence ofEmerging microbes & infections · 2023Review
- Cell and Tissue Tropism of Brucella spp.Infection and immunity · 2023Review
- Histopathologic and immunohistochemical findings in the placentas and fetuses of domestic swine naturally infected withJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2023Article
- Seroprevalence of Brucella canis in canines from a dog shelter in Bogotá, ColombiaBiomedica : revista del Instituto Nacional de Salud · 2021Article
- Uncovering the Hidden Credentials ofMicrobiology and molecular biology reviews : MMBR · 2021Review
- Review
- Canine Brucellosis: An Update.Frontiers in veterinary science · 2021Review
- The Role of Neutrophils in Brucellosis.Microbiology and molecular biology reviews : MMBR · 2020Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brucella canis infection is an underdiagnosed zoonotic disease. Knowledge about perinatal brucellosis in dogs is extremely limited, although foetuses and neonates are under risk of infection due to vertical transmission. In this study, immunohistochemistry was used to determine tissue distribution and cell tropism of B. canis in canine foetuses and neonates. Diagnosis of B. canis in tissues of naturally infected pups was based on PCR and sequencing of amplicons, bacterial isolation, and immunohistochemistry, whose specificity was confirmed by laser capture microdissection. PCR positivity among 200 puppies was 21%, and nine isolates of B. canis were obtained. Tissues from 13 PCR-positive puppies (4 stillborn and 9 neonates) presented widespread immunolabeling. Stomach, intestines, kidney, nervous system, and umbilicus were positive in all animals tested. Other frequently infected organs included the liver (92%), lungs (85%), lymph nodes (69%), and spleen (62%). Immunolabeled coccobacilli occurred mostly in macrophages, but they were also observed in erythrocytes, epithelial cells of gastrointestinal mucosa, renal tubules, epidermis, adipocytes, choroid plexus, ependyma, neuroblasts, blood vessels endothelium, muscle cells, and in the intestinal lumen. These results largely expand our knowledge about perinatal brucellosis in the dog, clearly demonstrating a pantropic distribution of B. canis in naturally infected foetuses and neonates.
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