ArticleScientific reports2018
Novel antibiofilm chemotherapies target nitrogen from glutamate and glutamine.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- Global Transcriptional Differences inAntibiotics (Basel, Switzerland) · 2026Article
- Role of NaCl and Glutamine on Biofilm Production fromMicroorganisms · 2025Article
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- In Vitro Evaluation of the Antibacterial Effect and Influence on the Bacterial Biofilm Formation of Glutamic Acid and Some Structural Analogues.Antibiotics (Basel, Switzerland) · 2025Article
- Mechanisms of antibiofilm compounds JG-1 and M4 across multiple species: alterations of protein interactions essential to biofilm formation.Frontiers in cellular and infection microbiology · 2025Article
- The Utilization ofMicroorganisms · 2024Article
- Cooperative growth in microbial communities is a driver of multistability.Nature communications · 2024Article
- Acetylation of xenogeneic silencer H-NS regulates biofilm development through the nitrogen homeostasis regulator in Shewanella.Nucleic acids research · 2024Article
- Competition for iron shapes metabolic antagonism between Bacillus subtilis and Pseudomonas marginalis.The ISME journal · 2024Article
- Article
- The metabolomic plasma profile of patients with Duchenne muscular dystrophy: providing new evidence for its pathogenesis.Orphanet journal of rare diseases · 2023Article
- Drug delivery strategies for antibiofilm therapy.Nature reviews. Microbiology · 2023Review
- Comparative Proteomic Analysis of Protein Patterns ofMicroorganisms · 2023Article
- GltS regulates biofilm formation in methicillin-resistant Staphylococcus aureus.Communications biology · 2022Article
- The roles of intracellular and extracellular calcium iniScience · 2022Article
- Structural basis for the helical filament formation of Escherichia coli glutamine synthetase.Protein science : a publication of the Protein Society · 2022Article
- Article
- Emerging Roles of Functional Bacterial Amyloids in Gene Regulation, Toxicity, and Immunomodulation.Microbiology and molecular biology reviews : MMBR · 2020Review
- Role of Glutamate Synthase in Biofilm Formation by Bacillus subtilis.Journal of bacteriology · 2020Article
- Microbial Metabolic Genes Crucial forFrontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteria in nature often reside in differentiated communities termed biofilms, which are an active interphase between uni-cellular and multicellular life states for bacteria. Here we demonstrate that the development of B. subtilis biofilms is dependent on the use of glutamine or glutamate as a nitrogen source. We show a differential metabolic requirement within the biofilm; while glutamine is necessary for the dividing cells at the edges, the inner cell mass utilizes lactic acid. Our results indicate that biofilm cells preserve a short-term memory of glutamate metabolism. Finally, we establish that drugs that target glutamine and glutamate utilization restrict biofilm development. Overall, our work reveals a spatial regulation of nitrogen and carbon metabolism within the biofilm, which contributes to the fitness of bacterial complex communities. This acquired metabolic division of labor within biofilm can serve as a target for novel anti-biofilm chemotherapies.
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Registered trials
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