ArticleEmerging microbes & infections2018
Sensitive detection of Treponema pallidum DNA from the whole blood of patients with syphilis by the nested PCR assay.
Article in Emerging microbes & infections, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 36 citations in OpenAlex.
- Mapping the distribution of yaws and Haemophilus ducreyi in the western north region of Ghana.BMC infectious diseases · 2025Observational
- Detection of Treponema pallidum tpp47 DNA in clinical samples of syphilis patients.European journal of medical research · 2025Article
- Advances in Syphilis Diagnostics to Address the 21st-Century Epidemic.Clinical chemistry · 2025Review
- 405 nm light microbicidal efficacy on Treponema pallidum spiked in ex vivo human platelets.Scientific reports · 2025Article
- Research Advances in Clinical Strategies and Preclinical Models for Syphilis Relapse.Infection and drug resistance · 2025Review
- Prospective evaluation of non-invasive saliva specimens for the diagnosis of syphilis and molecular surveillance ofJournal of clinical microbiology · 2024Article
- Loop-Mediated Isothermal Amplification Assay Using Gold Nanoparticles for DetectingDiagnostics (Basel, Switzerland) · 2024Article
- Epidemic of multiple Treponema pallidum strains in men who have sex with men in Japan: efficient multi-locus sequence typing scheme and indicator biomarkers.AIDS research and therapy · 2024Article
- Multiplex Loop-Mediated Isothermal Amplification Coupled Lateral Flow Assay for Point-of-Care Detection of Syphilis.Indian journal of microbiology · 2024Article
- CDC Laboratory Recommendations for Syphilis Testing, United States, 2024.MMWR. Recommendations and reports : Morbidity and mortality weekly report. Recommendations and reports · 2024Article
- Refining Timely Diagnosis of Early Syphilis by Using Treponema pallidum PCR or IgM Immunoblotting Next to Conventional Serology for Syphilis.Journal of clinical microbiology · 2023Article
- Development and clinical validation of a one-step pentaplex real-time reverse transcription PCR assay for detection of hepatitis virus B, C, E, Treponema pallidum, and a human housekeeping gene.BMC infectious diseases · 2023Article
- Molecular Characteristics of Macrolide Resistance inInfection and drug resistance · 2023Article
- Appraisal of vitreous syphilis antibody as a novel biomarker for the diagnosis of syphilitic uveitis: a prospective case-control study.Eye (London, England) · 2023Article
- Detection of Treponema pallidum DNA During Early Syphilis Stages in Peripheral Blood, Oropharynx, Ano-Rectum and Urine as a Proxy for Transmissibility.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2022Article
- Quantified Detection of Treponema pallidum DNA by PCR Assays in Urine and Plasma of Syphilis Patients.Microbiology spectrum · 2022Article
- Detection of Treponema pallidum in whole blood samples of patients with syphilis by the polymerase chain reaction.Revista do Instituto de Medicina Tropical de Sao Paulo · 2022Article
- A New Specimen for Syphilis Diagnosis: Evidence by High Loads of Treponema pallidum DNA in Saliva.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Analytical Performance Characteristics of a New Transcription-Mediated Amplification Assay for Treponema pallidum.Journal of clinical microbiology · 2021Article
- Laboratory Diagnostic Tools for Syphilis: Current Status and Future Prospects.Frontiers in cellular and infection microbiology · 2020Review
Corrections and comments
- Erratum issuedCorrection.2020
Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this work was to investigate the application of the nested PCR assay for the detection of Treponema pallidum (TP) DNA from the blood of patients with different stages of syphilis. In this study, a nested PCR method targeting the Tpp47 and polA genes (Tpp47-Tp-PCR and polA-Tp-PCR) was developed to detect TP-DNA in whole blood samples collected from 262 patients with different stages of syphilis (84 primary syphilis, 97 secondary syphilis, and 81 latent syphilis patients). The PCR assay detected T. pallidum DNA in 53.6% and 62.9% of the patients with primary and secondary syphilis, respectively, which was much higher than the detection levels in patients with latent syphilis (7.4%) (both p < 0.001). For primary syphilis, a low RPR (0-16) was correlated with a higher detection rate of TP-DNA, whereas for secondary syphilis, the higher detection rate of blood TP-DNA was correlated with higher blood RPR titers (at or beyond 32). For latent syphilis, TP-DNA was only detectable by PCR in the early phase of the latent infection. Thus, blood RPR titers were correlated with the blood T. pallidum burden, but the correlations varied with primary and secondary syphilis. The results indicate that nested PCR is a sensitive method for detecting blood TP-DNA and is especially useful for detecting early syphilis including primary syphilis and secondary syphilis. The findings also suggest that the PCR assay may be used to complement other methods to enhance the diagnosis of syphilis.
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Registered trials
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