Evidence map›Paper›PMID 29738673›Full record

ArticleBioconjugate chemistry2018

Top-Down Chemoenzymatic Approach to Synthesizing Diverse High-Mannose N-Glycans and Related Neoglycoproteins for Carbohydrate Microarray Analysis.

Christian Toonstra, Lisa Wu, Chao Li, Denong Wang, Lai-Xi Wang

Open access · greenAbstract read
In one paragraph

Article in Bioconjugate chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 26 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Review
  9. Article
  10. Review
  11. Coronaviruses' sugar shields as vaccine candidates.Current trends in immunology · 2020
    Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Christian ToonstraDepartment of Chemistry and Biochemistry , University of Maryland , College Park , Maryland 20742 , United States.
Lisa WuTumor Glycomics Laboratory , SRI International Biosciences Division , Menlo Park , California 94025 , United States.
Chao LiDepartment of Chemistry and Biochemistry , University of Maryland , College Park , Maryland 20742 , United States.
Denong WangTumor Glycomics Laboratory , SRI International Biosciences Division , Menlo Park , California 94025 , United States.
Lai-Xi WangDepartment of Chemistry and Biochemistry , University of Maryland , College Park , Maryland 20742 , United States.ORCID 0000-0003-4293-5819
University of Maryland, College Park · USSRI International · US

Funding

Synthetic HIV Vaccine Targeting Glycopeptide Neutralizing EpitopesR01AI113896 · NIAID · UNIV OF MARYLAND, COLLEGE PARK · PI WANG, LAI-XI · 2014 to 2018
$2.3M
B-1 B cell responses to oligomannosyl antigens of HIV-1R56AI118464 · NIAID · SRI INTERNATIONAL · PI WANG, DENONG · 2015 to 2015
$884k
A T-independent pathway of B cell responses to oligomannosyl antigens of HIV-1R56AI108388 · NIAID · SRI INTERNATIONAL · PI WANG, DENONG · 2013 to 2013
$751k
IMMUNOGENIC SUGAR MOIETIES OF HCMVR21AI124068 · NIAID · SRI INTERNATIONAL · PI WANG, DENONG · 2017 to 2018
$521k
NIAID NIH HHS R01 AI113896NIAID NIH HHS R21 AI124068NIAID NIH HHS R56 AI108388NIAID NIH HHS R56 AI118464
6 · The paper itself

Abstract

High-mannose-type N-glycans are an important component of neutralizing epitopes on HIV-1 envelope glycoprotein gp120. They also serve as signals for protein folding, trafficking, and degradation in protein quality control. A number of lectins and antibodies recognize high-mannose-type N-glycans, and glycan array technology has provided an avenue to probe these oligomannose-specific proteins. We describe in this paper a top-down chemoenzymatic approach to synthesize a library of high-mannose N-glycans and related neoglycoproteins for glycan microarray analysis. The method involves the sequential enzymatic trimming of two readily available natural N-glycans, the Man

Indexed as

AnimalsBiocatalysisCattleChickensGlycine maxGlycoproteinsMannosePolysaccharidesSerum Albumin, BovineGlycoproteinsMannosePolysaccharidesSerum Albumin, Bovine

Identifiers

PMID29738673
PMCPMC6013400
OpenAlexW2802014813

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.