ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2018
Detection of toxoplasma-specific immunoglobulin G in human sera: performance comparison of in house Dot-ELISA with ECLIA and ELISA.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- The Relationship of Latent Toxoplasmosis and Cigarette Smoking: Seroprevalence, Risk Factor, and Case-Control Study in Fars Province, Southern Iran.Pathogens (Basel, Switzerland) · 2022Article
- Assessment of an In-House Enzyme-Linked Immunosorbent Assay and IgG Avidity Test Based on SAG1 and GRA7 Proteins for Discriminating between Acute and Chronic Toxoplasmosis in Humans.Journal of clinical microbiology · 2021Article
- Interference of hemolysis on the postmortem biochemical analysis of IgE by ECLIA.International journal of legal medicine · 2021Article
- Seroepidemiological study of toxoplasmosis in women referred to a pre-marriage counseling center in Alborz Province, Iran.BMC research notes · 2021Article
- An advanced molecularly imprinted electrochemical sensor for the highly sensitive and selective detection and determination of Human IgG.Bioelectrochemistry (Amsterdam, Netherlands) · 2021Article
- New prevalence surveillance ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2019Article
- Development, optimization, and validation of an in-house Dot-ELISA rapid test based on SAG1 and GRA7 proteins for serological detection ofInfection and drug resistance · 2019Article
- The relation of serum prolactin levels andInternational journal of general medicine · 2019Article
- The relation of secondary sex ratio and miscarriage history with Toxoplasma gondii infection.BMC infectious diseases · 2018Article
- The association of latent toxoplasmosis and level of serum testosterone in humans.BMC research notes · 2018Article
- Curcumin nanoemulsion as a novel chemical for the treatment of acute and chronic toxoplasmosis in mice.International journal of nanomedicine · 2018Article
- Associations betweenIranian journal of parasitologyArticle
- The Therapeutic Efficacy of Zinc Oxide Nanoparticles on Acute Toxoplasmosis in BALB/c Mice.Iranian journal of parasitologyArticle
- Current and Emerging Techniques for Diagnosis of Toxoplasmosis in Pregnancy: A Narrative Review.Iranian journal of parasitologyReview
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
In the current study, performance of electrochemiluminescence immunoassay (ECLIA) in detection of anti-toxoplasma IgG in human sera was compared with that of enzyme-linked immunosorbent assay (ELISA). Furthermore, performance of an in house Dot-ELISA in detection of anti-toxoplasma IgG was compared with that of ECLIA and ELISA. In total, 219 human sera were tested to detect anti-toxoplasma IgG using Dynex DS2® and Roche Cobas® e411 Automated Analyzers. Discordant results rechecked using immunofluorescence assay (IFA). Then, sera were used in an in house Dot-ELISA to assess toxoplasma-specific IgG. Of the 219 samples, two samples were found undetermined using ECLIA but reactive using ELISA. Using IFA, the two sera were reported unreactive. Furthermore, two samples were found reactive using ECLIA and unreactive using ELISA. These samples were reported reactive using IFA. The overall agreement for the two former methods was 98% (rZ0.98.1; P < 0.001). The intrinsic parameters calculated for in house Dot-ELISA included sensitivity of 79.5, specificity of 78.2, and accuracy of 78.9%, compared to ECLIA and ELISA. Positive and negative predictive values included 82.9 and 74.2%, respectively. A 100% sensitivity was found in in house Dot-ELISA for highly reactive sera in ECLIA and ELISA. ECLIA is appropriate for the first-line serological screening tests and can replace ELISA due to high speed, sensitivity, and specificity, particularly in large laboratories. Dot-ELISA is a rapid, sensitive, specific, cost-effective, user-friendly, and field-portable technique and hence can be used for screening toxoplasmosis, especially in rural fields or less equipped laboratories.
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