Evidence map›Paper›PMID 29735999›Full record

ArticleNature methods2018

Single-shot super-resolution total internal reflection fluorescence microscopy.

Min Guo, Panagiotis Chandris, John Paul Giannini, Adam J Trexler, Robert Fischer, Jiji Chen, Harshad D Vishwasrao, Ivan Rey-Suarez, Yicong Wu, Xufeng Wu and 5 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
10.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 76 citations in OpenAlex.

  1. Article
  2. Review
  3. Exploring Biophysics at the Membrane with Single-Molecule TIRF Microscopy.Advances in experimental medicine and biology · 2026
    Review
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Imaging nanoscale axial dynamics at the basal plasma membrane.The international journal of biochemistry & cell biology · 2023
    Review
  11. Super-Resolution Microscopy to Study Interorganelle Contact Sites.International journal of molecular sciences · 2022
    Review
  12. Article
  13. Review
  14. Article
  15. Review
  16. Article
  17. Super-Resolution Live Cell Microscopy of Membrane-Proximal Fluorophores.International journal of molecular sciences · 2020
    Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 2 institutions in 1 country.

Min GuoSection on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA. min.guo@nih.gov.ORCID http://orcid.org/0000-0002-2093-8771
Panagiotis ChandrisSection on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
John Paul GianniniSection on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
Adam J TrexlerNational Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
Robert FischerNational Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
Jiji ChenAdvanced Imaging and Microscopy Resource, National Institutes of Health, Bethesda, MD, USA.
Harshad D VishwasraoAdvanced Imaging and Microscopy Resource, National Institutes of Health, Bethesda, MD, USA.
Ivan Rey-SuarezSection on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
Yicong WuSection on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
Xufeng WuNational Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
Clare M WatermanNational Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
George H PattersonSection on Biophotonics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
Arpita UpadhyayaDepartment of Physics and Institute for Physical Science and Technology, University of Maryland, College Park, MD, USA.
Justin W TaraskaNational Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.ORCID http://orcid.org/0000-0001-5355-9535
Hari ShroffSection on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
National Institutes of Health · USUniversity of Maryland, College Park · US

Funding

Architecture and control of vesicle fusion in excitable cellsZIAHL006098 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI TARASKA, JUSTIN · 2011 to 2025
$25.5M
Section on High Resolution Optical ImagingZIAEB000074 · NIBIB · NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING · PI SHROFF, HARI · 2010 to 2022
$15.2M
BiophotonicsZIAEB000071 · NIBIB · NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING · PI PATTERSON, GEORGE · 2010 to 2022
$9.7M
Intramural NIH HHS Z99 EB999999
6 · The paper itself

Abstract

We combined instant structured illumination microscopy (iSIM) with total internal reflection fluorescence microscopy (TIRFM) in an approach referred to as instant TIRF-SIM, thereby improving the lateral spatial resolution of TIRFM to 115 ± 13 nm without compromising speed, and enabling imaging frame rates up to 100 Hz over hundreds of time points. We applied instant TIRF-SIM to multiple live samples and achieved rapid, high-contrast super-resolution imaging close to the coverslip surface.

Indexed as

Cell Line, TumorHumansMicroscopy, FluorescenceMicrotubulesOsteosarcomarab11 GTP-Binding Proteinsrab GTP-Binding Proteinsrab11 GTP-Binding Proteinsrab GTP-Binding Proteins

Identifiers

PMID29735999
PMCPMC7470603
OpenAlexW2751042822

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.