Evidence map›Paper›PMID 29735997›Full record

ArticleNature methods2018

Discovery of proteins associated with a predefined genomic locus via dCas9-APEX-mediated proximity labeling.

Samuel A Myers, Jason Wright, Ryan Peckner, Brian T Kalish, Feng Zhang, Steven A Carr

Abstract read
In one paragraph

Article in Nature methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 123 papers.

0numbers the graph read from it
0cells of the map it votes in
123citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

123 citing papers in PubMed.

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  18. Overcoming the eIF2α Brake in Human Cell-Derived Translation Systems.bioRxiv : the preprint server for biology · 2025
    Article
  19. Article
  20. Review

63 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Samuel A MyersBroad Institute of MIT and Harvard, Cambridge, MA, USA. sammyers@broadinstitute.org.ORCID http://orcid.org/0000-0001-7636-0887
Jason WrightBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Ryan PecknerBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Brian T KalishDepartment of Neurobiology, Harvard Medical School, Boston, MA, USA.
Feng ZhangBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Steven A CarrBroad Institute of MIT and Harvard, Cambridge, MA, USA. scarr@broad.mit.edu.ORCID http://orcid.org/0000-0002-7203-4299

Funding

Proteo-genomic Discovery, Prioritization and Verification of Cancer BiomarkersU24CA160034 · NCI · BROAD INSTITUTE, INC. · PI CARR, STEVEN A, PAULOVICH, AMANDA G · 2011 to 2016
$16.2M
Center for Cell CircuitsRM1HG006193 · NHGRI · BROAD INSTITUTE, INC. · PI BLAINEY, PAUL CLARK, HACOHEN, NIR · 2016 to 2020
$14.9M
Measuring Cancer Biomarker Candidates by Targeted MS and Ab EnrichmentU24CA126476 · NCI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI CARR, STEVEN A · 2006 to 2010
$14.6M
Programmable RNA-targeting toolsR01HG009761 · NHGRI · BROAD INSTITUTE, INC. · PI ZHANG, FENG · 2017 to 2024
$7.5M
Center of Excellence for High Throughput Proteogenomic CharacterizationU24CA210986 · NCI · BROAD INSTITUTE, INC. · PI CARR, STEVEN A, GILLETTE, MICHAEL A · 2016 to 2020
$6.4M
Massively-parallel functional interrogation of psychiatric geneticsR01MH110049 · NIMH · BROAD INSTITUTE, INC. · PI ZHANG, FENG · 2015 to 2019
$5.5M
NCI NIH HHS U24 CA126476NCI NIH HHS U24 CA160034NCI NIH HHS U24 CA210986NHGRI NIH HHS R01 HG009761NHGRI NIH HHS RM1 HG006193NIMH NIH HHS R01 MH110049
6 · The paper itself

Abstract

Regulation of gene expression is primarily controlled by changes in the proteins that occupy genes' regulatory elements. We developed genomic locus proteomics (GLoPro), in which we combine CRISPR-based genome targeting, proximity labeling, and quantitative proteomics to discover proteins associated with a specific genomic locus in native cellular contexts.

Indexed as

Chromatin ImmunoprecipitationCRISPR-Associated Protein 9DNA-(Apurinic or Apyrimidinic Site) LyaseEndonucleasesGenomeGenomicsHEK293 CellsHumansMultifunctional EnzymesPolymerase Chain ReactionProtein EngineeringAPEX2 protein, humanCRISPR-Associated Protein 9DNA-(Apurinic or Apyrimidinic Site) LyaseEndonucleasesMultifunctional Enzymes

Identifiers

PMID29735997
PMCPMC6202184

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.