ArticlePhilosophical transactions of the Royal Society of London. Series B, Biological sciences2018
Substrate protein dependence of GroEL-GroES interaction cycle revealed by high-speed atomic force microscopy imaging.
Article in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed.
- The mitochondrial intermembrane space protein mitofissin drives mitochondrial fission required for mitophagy.Molecular cell · 2023Article
- BioAFMviewer: An interactive interface for simulated AFM scanning of biomolecular structures and dynamics.PLoS computational biology · 2020Article
- Reconciling the controversy regarding the functional importance of bullet- and football-shaped GroE complexes.The Journal of biological chemistry · 2019Article
- Allostery and molecular machines.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Article
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2 authors.
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Abstract
A double-ring-shaped tetradecameric GroEL complex assists proper protein folding in cooperation with the cochaperonin GroES. The dynamic GroEL-GroES interaction reflects the allosteric intra- and inter-ring communications and the chaperonin reaction. Therefore, revealing this dynamic interaction is essential to understanding the allosteric communications and the operation mechanism of GroEL. Nevertheless, how this interaction proceeds in the chaperonin cycle has long been controversial. Here, we directly image the dynamic GroEL-GroES interaction under conditions with and without foldable substrate protein using high-speed atomic force microscopy. Then, the imaging results obtained under these conditions and our previous results in the presence of unfoldable substrate are compared. The molecular movies reveal that the entire reaction pathway is highly complicated but basically identical irrespective of the substrate condition. A prominent (but moderate) difference is in the population distribution of intermediate species: symmetric GroEL : GroES
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