ArticlePlant biotechnology journal2019
Targeting intracellular transport combined with efficient uptake and storage significantly increases grain iron and zinc levels in rice.
Article in Plant biotechnology journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 78 citations in OpenAlex.
- CRISPR/Cas-based genome editing for enhancing grain quality traits in rice (Plant signaling & behavior · 2026Review
- Iron and Other Metal Ions in Human Health and Disease.MedComm · 2026Review
- Machine learning-guided multi-omics suggests iron-dependent hormonal signaling drives root morphological plasticity in wheat under temperature stress.The New phytologist · 2026Article
- Review
- Engineering of nicotianamine synthesis enhances cadmium mobility in plants and results in higher seed cadmium concentrations.The Plant journal : for cell and molecular biology · 2025Article
- Strategies and bibliometric analysis of legumes biofortification to address malnutrition.Planta · 2024Review
- New insights into QTNs and potential candidate genes governing rice yield via a multi-model genome-wide association study.BMC plant biology · 2024Article
- Proof of concept and early development stage of market-oriented high iron and zinc rice expressing dicot ferritin and rice nicotianamine synthase genes.Scientific reports · 2023Article
- CRISPR-mediated promoter editing of aFrontiers in genome editing · 2023Article
- Biofortification to avoid malnutrition in humans in a changing climate: Enhancing micronutrient bioavailability in seed, tuber, and storage roots.Frontiers in plant science · 2023Review
- Genetic Approaches for Iron and Zinc Biofortification and Arsenic Decrease in Oryza sativa L. Grains.Biological trace element research · 2022Review
- Mining of Potential Gene Resources for Breeding Nutritionally Improved Maize.Plants (Basel, Switzerland) · 2022Review
- A tale of two metals: Biofortification of rice grains with iron and zinc.Frontiers in plant science · 2022Review
- Modification of storage proteins in the barley grain increases endosperm zinc and iron under both normal and elevated atmospheric COPhysiologia plantarum · 2022Article
- A promoter toolbox for tissue-specific expression supporting translational research in cassava (Frontiers in plant science · 2022Article
- Strategies and Bottlenecks in Hexaploid Wheat to Mobilize Soil Iron to Grains.Frontiers in plant science · 2022Review
- Potato biofortification: an effective way to fight global hidden hunger.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
- Evolutionarily conserved hierarchical gene regulatory networks for plant salt stress response.Nature plants · 2021Article
- Characterization of zinc solubilization potential of arsenic tolerant Burkholderia spp. isolated from rice rhizospheric soil.World journal of microbiology & biotechnology · 2021Article
- Multiplying the efficiency and impact of biofortification through metabolic engineering.Nature communications · 2020Review
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3 authors at 1 institution in 1 country.
Funding
Abstract
Rice, a staple food for more than half of the world population, is an important target for iron and zinc biofortification. Current strategies mainly focus on the expression of genes for efficient uptake, long-distance transport and storage. Targeting intracellular iron mobilization to increase grain iron levels has not been reported. Vacuole is an important cell compartment for iron storage and the NATURAL RESISTANCE ASSOCIATED MACROPHAGE PROTEIN (NRAMP) family of transporters export iron from vacuoles to cytosol when needed. We developed transgenic Nipponbare rice lines expressing AtNRAMP3 under the control of the UBIQUITIN or rice embryo/aleurone-specific 18-kDa Oleosin (Ole18) promoter together with NICOTIANAMINE SYNTHASE (AtNAS1) and FERRITIN (PvFER), or expressing only AtNRAMP3 and PvFER together. Iron and zinc were increased close to recommended levels in polished grains of the transformed lines, with maximum levels when AtNRAMP3, AtNAS1 and PvFER were expressed together (12.67 μg/g DW iron and 45.60 μg/g DW zinc in polished grains of line NFON16). Similar high iron and zinc levels were obtained in transgenic Indica IR64 lines expressing the AtNRAMP3, AtNAS1 and PvFER cassette (13.65 μg/g DW iron and 48.18 μg/g DW zinc in polished grains of line IR64_1), equalling more than 90% of the recommended iron increase in rice endosperm. Our results demonstrate that targeting intracellular iron stores in combination with iron and zinc transport and endosperm storage is an effective strategy for iron biofortification. The increases achieved in polished IR64 grains are of dietary relevance for human health and a valuable nutrition trait for breeding programmes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.