ArticleJournal of molecular biology2018
Characterizing Lysine Acetylation of Isocitrate Dehydrogenase in Escherichia coli.
Article in Journal of molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 51 citations in OpenAlex.
- Acetyl-Phosphate Dependent Protein Acetylation in Neisseria gonorrhoeae.MicrobiologyOpen · 2026Article
- Functional consequences of lysine acetylation of phosphofructokinase isozymes.The FEBS journal · 2025Article
- Article
- Efficiency of acetate-based isopropanol synthesis in Escherichia coli W is controlled by ATP demand.Biotechnology for biofuels and bioproducts · 2024Article
- Lysine Phoshoglycerylation Is Widespread in Bacteria and Overlaps with Acylation.Microorganisms · 2024Article
- Noncanonical Amino Acids in Biocatalysis.Chemical reviews · 2024Review
- The acetylation of MDH1 and IDH1 is associated with energy metabolism in acute liver failure.iScience · 2024Article
- Orthogonal Translation for Site-Specific Installation of Post-translational Modifications.Chemical reviews · 2024Review
- Bacteria employ lysine acetylation of transcriptional regulators to adapt gene expression to cellular metabolism.Nature communications · 2024Article
- Acetylomics reveals an extensive acetylation diversity withinmicroLife · 2024Article
- Characterizing lysine acetylation of glucokinase.Protein science : a publication of the Protein Society · 2024Article
- Bacterial protein acetylation: mechanisms, functions, and methods for study.Frontiers in cellular and infection microbiology · 2024Review
- Molecular Typing ofJournal of pharmacy & bioallied sciences · 2023Article
- Studying lysine acetylation of citric acid cycle enzymes by genetic code expansion.Molecular microbiology · 2023Review
- Acetylation of Cyclic AMP Receptor Protein by Acetyl Phosphate Modulates Mycobacterial Virulence.Microbiology spectrum · 2023Article
- Modulation of plant acetyl-CoA synthetase activity by post-translational lysine acetylation.Frontiers in molecular biosciences · 2023Article
- Regulation of the pyrimidine biosynthetic pathway by lysine acetylation of E. coli OPRTase.The FEBS journal · 2023Article
- Lysine acetylation decreases enzyme activity and protein level ofEngineering microbiology · 2022Article
- Acetylation of Lactate Dehydrogenase Negatively Regulates the Acidogenicity of Streptococcus mutans.mBio · 2022Article
- Lysine acetylation ofFrontiers in bioengineering and biotechnology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The Escherichia coli isocitrate dehydrogenase (ICDH) is one of the tricarboxylic acid cycle enzymes, playing key roles in energy production and carbon flux regulation. E. coli ICDH was the first bacterial enzyme shown to be regulated by reversible phosphorylation. However, the effect of lysine acetylation on E. coli ICDH, which has no sequence similarity with its counterparts in eukaryotes, is still unclear. Based on previous studies of E. coli acetylome and ICDH crystal structures, eight lysine residues were selected for mutational and kinetic analyses. They were replaced with acetyllysine by the genetic code expansion strategy or substituted with glutamine as a classic approach. Although acetylation decreased the overall ICDH activity, its effects were different site by site. Deacetylation tests demonstrated that the CobB deacetylase could deacetylate ICDH both in vivo and in vitro, but CobB was only specific for lysine residues at the protein surface. On the other hand, ICDH could be acetylated by acetyl-phosphate chemically in vitro. And in vivo acetylation tests indicated that the acetylation level of ICDH was correlated with the amounts of intracellular acetyl-phosphate. This study nicely complements previous proteomic studies to provide direct biochemical evidence for ICDH acetylation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.