ArticleJournal of visualized experiments : JoVE2018
A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 27 citations in OpenAlex.
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- The local-scale populations reveal cryptic processes occurring in the general population of Schizophyllum commune fungus.Scientific reports · 2025Article
- Identifying SSR/InDel loci related to tobacco bacterial wilt resistance using association mapping.Heliyon · 2024Article
- Analysis of genetic diversity and population structure of some Ethiopian barley (Hordeum vulgare L.) accessions using SSR markers.PloS one · 2024Article
- Genetic variation and structure of endemic and endangered wild celery (Frontiers in plant science · 2024Article
- Identification and genetic diversity analysis of hybrid offspring of azalea based on EST-SSR markers.Scientific reports · 2022Article
- Variability in waxy (Wx) allele, in-vitro starch digestibility, glycemic response and textural behaviour of popular Northern Himalayan rice varieties.Scientific reports · 2021Article
- Transcriptome characterization and generation of marker resource for Himalayan vulnerable species, Ulmus wallichiana.Molecular biology reports · 2021Article
- Genetic variability and population structure of Ethiopian chickpea (Cicer arietinum L.) germplasm.PloS one · 2021Article
- Postnatal Growth Restriction in Mice Alters Cardiac Protein Composition and Leads to Functional Impairment in Adulthood.International journal of molecular sciences · 2020Article
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Simple Sequence Repeat (SSR) is one of the most effective markers used in plant and animal genetic research and molecular breeding programs. Silver staining is a widely used method for the detection of SSR markers in a polyacrylamide gel. However, conventional protocols for silver staining are technically demanding and time-consuming. Like many other biological laboratory techniques, silver staining protocols have been steadily optimized to improve detection efficiency. Here, we report a simplified silver staining method that significantly reduces reagent costs and enhances the detection resolution and picture clarity. The new method requires two major steps (impregnation and development) and three reagents (silver nitrate, sodium hydroxide, and formaldehyde), and only 7 min of processing for a non-denaturing polyacrylamide gel. Compared to previously reported protocols, this new method is easier, quicker and uses fewer chemical reagents for SSR detection. Therefore, this simple, low-cost, and effective silver staining protocol will benefit genetic mapping and marker-assisted breeding by a quick generation of SSR marker data.
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