Evidence map›Paper›PMID 29732415›Full record

ArticleReactive oxygen species (Apex, N.C.)2017

In Vivo Bioluminescence Imaging of Nuclear Factor kappaB Activation: A Valuable Model for Studying Inflammatory and Oxidative Stress in Live Mice.

Hong Zhu, Zhenquan Jia, Michael A Trush, Y Robert Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Reactive oxygen species (Apex, N.C.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. 3Reactive oxygen species (Apex, N.C.) · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Hong ZhuCampbell University School of Osteopathic Medicine, Buies Creek, NC 27506, USA.
Zhenquan JiaCampbell University School of Osteopathic Medicine, Buies Creek, NC 27506, USA.
Michael A TrushDepartment of Environmental Health Sciences, The Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205, USA.
Y Robert LiCampbell University School of Osteopathic Medicine, Buies Creek, NC 27506, USA.

Funding

Cruciferous Dithiolethiones for Chronic Heart Failure: Signaling MechanismsR15CA192936 · NCI · CAMPBELL UNIVERSITY · PI LI, YUNBO · 2014 to 2014
$469k
Novel Carbon Nanodots against Vascular InflammationR15HL129212 · NHLBI · UNIVERSITY OF NORTH CAROLINA GREENSBORO · PI JIA, ZHENQUAN · 2016 to 2016
$455k
NCI NIH HHS R15 CA192936NHLBI NIH HHS R15 HL129212
6 · The paper itself

Abstract

The nuclear factor kappaB (NF-κB) is a redox-sensitive transcription factor that plays a critical role in inflammation among other biological functions. This ROS Protocol article describes an in vivo bioluminescence imaging assay for assessing NF-κB activation using the commercially available transgenic mice carrying NF-κB response element-luciferase reporter gene (NF-κB-RE-Luc). Using the highly sensitive Berthold NightOwl LB981 in vivo bioluminescence imaging system, we are able to visualize the NF-κB activation in live mice under basal conditions, suggesting constitutive activation of NF-κB as a part of its fundamental biology. Treatment of mice with lipopolysaccharides (LPS) results in a drastic increase in bioluminescence, proving the validity of the model in assessing inflammatory stress. Treatment of mice with 3

Indexed as

3H-1,2-Dithiole-3-thioneInflammatory stressIn vivo bioluminescence imagingNuclear factor kappaBOxidative stress

Identifiers

PMID29732415
PMCPMC5931218

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.