ArticleChemical science2018
Near-infrared triggered strand displacement amplification for MicroRNA quantitative detection in single living cells.
Article in Chemical science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 98 citations in OpenAlex.
- A Novel miRNA Detection Method Using Loop-Mediated Isothermal Amplification.International journal of analytical chemistry · 2023Article
- Electrochemiluminescence resonance energy transfer biosensing platform between g-CBiosensors & bioelectronics · 2022Article
- Programmable mismatch-fueled high-efficiency DNA signal amplifier.Chemical science · 2022Article
- A controlled T7 transcription-driven symmetric amplification cascade machinery for single-molecule detection of multiple repair glycosylases.Chemical science · 2021Article
- Programmable mismatch-fueled high-efficiency DNA signal converter.Chemical science · 2020Article
- An intramolecular catalytic hairpin assembly on a DNA tetrahedron for mRNA imaging in living cells: improving reaction kinetics and signal stability.Chemical science · 2019Article
- Dandelion flower-like micelles.Chemical science · 2019Article
- Research advances in the detection of miRNA.Journal of pharmaceutical analysis · 2019Review
- Substrate specificity-enabled terminal protection for direct quantification of circulating MicroRNA in patient serums.Chemical science · 2019Article
- Near-Infrared Light-Activated DNA-Agonist Nanodevice for Nongenetically and Remotely Controlled Cellular Signaling and Behaviors in Live Animals.Nano letters · 2019Article
- Probing cytoplasmic and nuclear microRNAs in single living cellsChemical science · 2018Article
- DNA Nanotechnology for Cancer Diagnosis and Therapy.International journal of molecular sciences · 2018Review
- Catalytic hairpin assembly gel assay for multiple and sensitive microRNA detection.Theranostics · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As an important modulator of gene expression, microRNA (miRNA) has been described as a promising biomarker for the early diagnosis of cancers. A non-invasive method for real-time sensitive imaging and monitoring of miRNA in living cells is in urgent demand. Although some amplified methods have been developed, few can be programmed to assemble single intelligent nanostructures to realize sensitive intracellular miRNA detection without extra addition of an enzyme or catalytic fuel. Herein, two programmable oligonucleotide hairpin probe functionalized gold nanorods (THP-AuNRs) were designed to develop a near-infrared (NIR) laser triggered target strand displacement amplification (SDA) approach for sensitive miRNA imaging quantitative analysis in single living cells and multicellular tumor spheroids (MCTSs). Such a NIR-triggered SDA strategy achieves facile and sensitive monitoring of a model oncogenic miRNA-373 in various cancer lines and MCTS simulated tumor tissue. Notably, using a linear regression equation derived from miRNA mimics, a quantitative method of miRNA in single living cells was realized due to the high sensitivity. This provides a new way for sensitive real-time monitoring of intracellular miRNA, and may be promising for miRNA-based biomedical applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.